|Tested species reactivity||Bovine, Dog, Human, Mouse, Sheep, Rat|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide between to amino acids 100-200 of the human HMGB1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris glycine|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunocytochemistry (ICC)||0.05 µg/ml|
|Immunofluorescence (IF)||0.05 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:250|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is expected to cross react with porcine, equine and rabbit samples based on 100% sequence homology.
Suggested positive control: Hela whole cell extract, antigen standard for HMGB1 (transient overexpression lysate).
HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. These proteins (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain an N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of these proteins contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins.
HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. They may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
The presence of LC3B puncta and HMGB1 expression in malignant cells correlate with the immune infiltrate in breast cancer.
PA1-16926 was used in immunohistochemistry - paraffin section to assess the correlation with the immune infiltration in breast cancer due to the presence of HMGB1and LC3B puncta expression in malignant cells
|Ladoire S,Enot D,Senovilla L,Ghiringhelli F,Poirier-Colame V,Chaba K,Semeraro M,Chaix M,Penault-Llorca F,Arnould L,Poillot ML,Arveux P,Delaloge S,Andre F,Zitvogel L,Kroemer G||Autophagy (12:864)||2016|
|Not Applicable||Not Cited||
Combined evaluation of LC3B puncta and HMGB1 expression predicts residual risk of relapse after adjuvant chemotherapy in breast cancer.
PA1-16926 was used in immunohistochemistry - paraffin section to predict residual risk of relapse after adjuvant chemotherapy in breast cancer by combined evaluation of HMGB1 and LC3B puncta
|Ladoire S,Penault-Llorca F,Senovilla L,Dalban C,Enot D,Locher C,Prada N,Poirier-Colame V,Chaba K,Arnould L,Ghiringhelli F,Fumoleau P,Spielmann M,Delaloge S,Poillot ML,Arveux P,Goubar A,Andre F,Zitvogel L,Kroemer G||Autophagy (11:1878)||2015|
Negative prognostic impact of regulatory T cell infiltration in surgically resected esophageal cancer post-radiochemotherapy.
PA1-16926 was used in immunohistochemistry - paraffin section to examine the immune responses in patients with esophageal carcinomas treated by neo-adjuvant radiochemotherapy
|Vacchelli E,Semeraro M,Enot DP,Chaba K,Poirier Colame V,Dartigues P,Perier A,Villa I,Rusakiewicz S,Gronnier C,Goéré D,Mariette C,Zitvogel L,Kroemer G||Oncotarget (6:20840)||2015|