Invitrogen

HMGCR Monoclonal Antibody (CL0259)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

1 Reference

View all (16) HMGCR antibodies

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Datasheet

Protocols

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HMGCR Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of HMGCR in human breast cancer using a HMGCR monoclonal antibody (Product # MA5-31335). The analysis shows moderate cytoplasmic immunoreactivity in tumor cells.

HMGCR Antibody in Western Blot (WB) — Western blot analysis of HMGCR by a HMGCR monoclonal antibody (Product # MA5-31335). Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line MCF-7 Lane 3: Human cell line HepG2.

HMGCR Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF-7 cells were transfected with, control siRNA, HMGCR siRNA probe #1, and HMGCR siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using anti-HMGCR Monoclonal Antibody (CL0259) (Product # MA5-31335) used at a dilution of 1 µg/mL. Anti-HMGCR was used as a loading control. {KD}

Product Details

MA5-31335

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	CL0259
Immunogen	Recombinant protein corresponding to Human HMGCR View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.6 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2786972

Product Specific Information

Immunogen sequence: HIPAYKLETL METHERGVSI RRQLLSKKLS EPSSLQYLPY RDYNYSLVMG ACCENVIGYM PIPVGVAGPL CLDEKEFQVP MATTEGCLVA

Target Information

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. Grik2, also known as glutamate receptor 6, belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. Recent reports have suggested that defects in the Grik2 protein may be associated with autosomal recessive mental retardation and possibly other neurological disorders such as schizophrenia. Numerous isoforms of Grik2 are known to exist and may be subject to RNA editing within the second transmembrane domain, which is thought to alter the properties of ion flow. This Grik2 antibody may exhibit some cross-reactivity to Grik3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mitochondrial respiratory chain dysfunction alters ER sterol sensing and mevalonate pathway activity.

The Journal of biological chemistry

Wall CTJ,Lefebvre G,Metairon S,Descombes P,Wiederkehr A,Santo-Domingo J

Published figure using HMGCR monoclonal antibody (Product # MA5-31335) in Western Blot

Tue Mar 01 00:00:00 EST 2022

Bioinformatics

Protein Aliases: 3-hydroxy-3-methylglutaryl CoA reductase (NADPH); 3-hydroxy-3-methylglutaryl-coenzyme A reductase; HMDH; HMG CoA reductase; HMG CoAR; HMG-CoA reductase; hydroxymethylglutaryl-CoA reductase; MGC103269

Gene Aliases: HMGCR; LDLCQ3

UniProt ID: (Human) P04035

Entrez Gene ID: (Human) 3156

Molecular Function: oxidoreductase reductase metabolite interconversion enzyme

Function(s)

cholesterol biosynthetic process ubiquinone metabolic process aging response to nutrient isoprenoid biosynthetic process visual learning negative regulation of striated muscle cell apoptotic process positive regulation of cardiac muscle cell apoptotic process coenzyme A metabolic process positive regulation of stress-activated MAPK cascade negative regulation of MAP kinase activity myoblast differentiation response to ethanol negative regulation of vasodilation positive regulation of skeletal muscle tissue development positive regulation of smooth muscle cell proliferation protein tetramerization oxidation-reduction process negative regulation of wound healing negative regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of ERK1 and ERK2 cascade

Process(es)

hydroxymethylglutaryl-CoA reductase (NADPH) activity protein binding hydroxymethylglutaryl-CoA reductase activity protein homodimerization activity coenzyme binding protein phosphatase 2A binding NADPH binding

HMGCR Monoclonal Antibody (CL0259)

Product Details

MA5-31335

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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