Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:3,000 | View 4 publications 4 publications |
Immunohistochemistry (IHC) |
- | View 3 publications 3 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100-1:1,000 | - |
Immunocytochemistry (ICC/IF) |
1:100-1:1,000 | - |
ELISA (ELISA) |
Assay-dependent | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Non-human primate, Rat |
Published species |
Human, Mouse, Sheep |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant fragment corresponding to a region within amino acids 1 and 278 of Human Heme Oxygenase 1 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.45 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7, with 20% glycerol |
Contains |
0.025% ProClin 300 |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2544814 |
Recommended positive controls: HeLa, Raw264.7, C2C12, rat spleen, HeLa (500 µM CoCl2 treatment for 24 hr).
Predicted reactivity: Mouse (81%), Rat (80%), Dog (85%), Pig (82%), Bovine (83%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; Heme oxygenase 1; Heme oxygenase 1 soluble form; hemoxygenase; HO-1; HSP32; P32 protein
Gene Aliases: bK286B10; D8Wsu38e; Hemox; Heox; HEOXG; Hmox; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32
UniProt ID: (Human) P09601, (Rat) P06762, (Mouse) P14901
Entrez Gene ID: (Human) 3162, (Rat) 24451, (Mouse) 15368
Molecular Function:
oxygenase
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