Invitrogen

HMOX1 Polyclonal Antibody

Product Image Gallery

HMOX1 Antibody — Published figure using HMOX1 polyclonal antibody (Product # PA5-27338)

HMOX1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Heme Oxygenase 1 showing staining in the nucleus and cytoplasm of A375 cells. A375 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Heme Oxygenase 1 polyclonal antibody (Product # PA5-27338) diluted at 1:500. Blue: Hoechst 33342 staining.

HMOX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Heme Oxygenase 1 in the cytoplasm of paraffin-embedded human renal carcinoma using a Heme Oxygenase 1 polyclonal antibody (Product # PA5-27338).

HMOX1 Antibody in Western Blot (WB) — Western blot analysis of Heme Oxygenase 1 using A) 30 µg BCL-1 whole cell lysate (B) 30 µg Raw264.7 whole cell lysate and C) 30 µg C2C12 whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Heme Oxygenase 1 polyclonal antibody (Product # PA5-27338) at a dilution of 1:1000.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:3000

Published Applications

Western Blot (WB)

See 1 publication below

Immunohistochemistry (IHC)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 278 of Human Heme Oxygenase 1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2544814

Product Specific Information

PA5-27338 targets Heme Oxygenase 1 in IHC (P) and WB applications and shows reactivity with Human and mouse samples.

The PA5-27338 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 278 of Human Heme Oxygenase 1.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 32-kD; bK286B10; heat shock protein; heat shock protein, 32-kD; heme oxygenase (decycling) 1; Heme oxygenase 1; hemoxygenase; HO-1; P32 protein

Gene Aliases: bK286B10; D8Wsu38e; Hemox; Hmox; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32

UniProt ID: (Human) P09601, (Mouse) P14901

Entrez Gene ID: (Human) 3162, (Mouse) 15368

Molecular Function: oxidoreductase oxygenase