Invitrogen

HMOX1 Polyclonal Antibody

Product Image Gallery

HMOX1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of HMOX1 was performed in Mock and treated HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HMOX1 Polyclonal Antibody (Product # PA5-27338) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

HMOX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of HMOX1 was performed in paraffin-embedded rat spleen tissue using HMOX1 Polyclonal Antibody (Product # PA5-27338) at a dilution of 1:500.

HMOX1 Antibody in Western Blot (WB) — Western blot analysis of HMOX1 was performed by separating 50 µg of mouse tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a HMOX1 Polyclonal Antibody (Product # PA5-27338) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 278 of Human Heme Oxygenase 1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.62 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2544814

Product Specific Information

Recommended positive controls: HeLa, Raw264.7, C2C12, rat spleen, HeLa (500 µM CoCl2 treatment for 24 hr).

Predicted reactivity: Mouse (81%), Rat (80%), Dog (85%), Pig (82%), Bovine (83%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; Heme oxygenase 1; hemoxygenase; HO-1; HSP32; P32 protein

Gene Aliases: bK286B10; D8Wsu38e; Hemox; Heox; HEOXG; Hmox; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32

UniProt ID: (Human) P09601, (Rat) P06762, (Mouse) P14901

Entrez Gene ID: (Human) 3162, (Rat) 24451, (Mouse) 15368

Molecular Function: oxygenase