Immunofluorescent analysis of HNF-1 alpha showing staining in the cytoplasm and nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a HNF-1 alpha polyclonal antibody (Product # PA5-22310) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 35 and 268 of HNF1 alpha (Uniprot ID#P20823)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-22310 targets HNF-1 alpha in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-22310 immunogen is a recombinant fragment corresponding to a region within amino acids 35 and 268 of HNF1 alpha (Uniprot ID#P20823).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.