|Tested species reactivity||Human, Rat|
|Published species reactivity||Non-human primate|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Baculovirus-expressed recombinant human HNF4-alpha (393-461 aa)|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||physiological saline|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay Dependent|
|ELISA (ELISA)||Assay Dependent|
|Gel Shift (GS)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effects of maternal nutrient restriction, intrauterine growth restriction, and glucocorticoid exposure on phosphoenolpyruvate carboxykinase-1 expression in fetal baboon hepatocytes in vitro.
417700 was used in immunocytochemistry and western blot to develop a cell culture system for fetal baboon hepatocytes and study phosphoenolpyruvate carboxykinase-.
|Li C,Shu ZJ,Lee S,Gupta MB,Jansson T,Nathanielsz PW,Kamat A||Journal of medical primatology (42:211)||2013|