Proteintech

HO-1/HMOX1 Polyclonal Antibody

Product Image Gallery

HO-1/HMOX1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HT-1080 cells using 10701-1-AP (HMOX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

HO-1/HMOX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 10701-1-AP ( HMOX1 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

HO-1/HMOX1 Antibody in Western Blot (WB) — HT-1080 cells were subjected to SDS PAGE followed by western blot with 10701-1-AP (HO-1/HMOX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

HO-1/HMOX1 Antibody in Immunoprecipitation (IP) — IP result of anti-HMOX1 (IP:10701-1-AP, 3ug; Detection:10701-1-AP 1:1000) with HeLa cells lysate 3000ug.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:20-1:200

Immunofluorescence (IF)

1:20-1:200

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Western Blot (WB)

1:500-1:2000

View 80 publications 80 publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Miscellaneous PubMed (Misc)

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HO-1/HMOX1 Fusion Protein Ag1190

Conjugate

Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; Heme oxygenase 1; hemoxygenase; HO-1; HSP32; P32 protein

Gene Aliases: bK286B10; D8Wsu38e; Hemox; Heox; HEOXG; Hmox; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32

UniProt ID: (Human) P09601, (Rat) P06762, (Mouse) P14901

Entrez Gene ID: (Human) 3162, (Rat) 24451, (Mouse) 15368

Molecular Function: oxidoreductase oxygenase