Proteintech

HO-1/HMOX1 Polyclonal Antibody

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HO-1/HMOX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 27282-1-AP (HO-1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

HO-1/HMOX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney tissue slide using 27282-1-AP (HO-1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

HO-1/HMOX1 Antibody in Western Blot (WB) — Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1/HMOX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

HO-1/HMOX1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1/HMOX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

HO-1/HMOX1 Antibody in Western Blot (WB) — Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

HO-1/HMOX1 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1/HMOX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

HO-1/HMOX1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1/HMOX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

HO-1/HMOX1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1/HMOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

HO-1/HMOX1 Antibody in Western Blot (WB) — A549 cells were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Western Blot (WB)

1:500-1:1000

View 13 publications 13 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Pig, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

HO-1/HMOX1 Fusion Protein Ag26172

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: heat shock protein, 32-kD; heme orygenase 1; heme oxygenase (decycling) 1; Heme oxygenase 1; hemoxygenase; HO-1; P32 protein

Gene Aliases: bK286B10; D8Wsu38e; Hemox; Hmox; HMOX1; HMOX1D; HO; HO-1; HO1; HSP32

UniProt ID: (Human) P09601, (Mouse) P14901

Entrez Gene ID: (Human) 3162, (Mouse) 15368

Molecular Function: oxidoreductase oxygenase

angiogenesis endothelial cell proliferation wound healing involved in inflammatory response negative regulation of leukocyte migration heme oxidation cellular iron ion homeostasis response to oxidative stress small GTPase mediated signal transduction excretion regulation of blood pressure cell death intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of muscle cell apoptotic process smooth muscle hyperplasia cellular response to nutrient negative regulation of mast cell cytokine production erythrocyte homeostasis low-density lipoprotein particle clearance regulation of transcription from RNA polymerase II promoter in response to iron cellular response to heat response to nicotine intracellular signal transduction heme catabolic process response to hydrogen peroxide positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of mast cell degranulation negative regulation of DNA binding negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of neuron apoptotic process regulation of transcription from RNA polymerase II promoter in response to oxidative stress response to estrogen positive regulation of chemokine biosynthetic process regulation of angiogenesis positive regulation of angiogenesis positive regulation of vasodilation positive regulation of smooth muscle cell proliferation negative regulation of smooth muscle cell proliferation regulation of sequence-specific DNA binding transcription factor activity protein homooligomerization iron ion homeostasis cellular response to arsenic-containing substance cellular response to cadmium ion cellular response to hypoxia negative regulation of extrinsic apoptotic signaling pathway via death domain receptors apoptotic process negative regulation of cell proliferation heme metabolic process oxidation-reduction process

Process(es)

heme oxygenase (decyclizing) activity phospholipase D activity signal transducer activity protein binding enzyme binding heme binding protein homodimerization activity metal ion binding oxidoreductase activity