Invitrogen

HOMER1 Polyclonal Antibody

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HOMER1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of HOMER1 in HepG2. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115374) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with Alexa Fluor 594 conjugated goat anti-rabbit IgG using a dilution of 1:600.

HOMER1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of HOMER1 in human lymph cancer tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115374) at a dilution of 1:100. Secondary staining was applied with HRP conjugated goat anti-rabbit.

HOMER1 Antibody in Western Blot (WB) — Western blot analysis of HOMER1 in human/mouse brain. Samples were incubated with polyclonal antibody (Product # PA5-115374).

Product Details

PA5-115374

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human HOMER1(Accession Q86YM7), corresponding to amino acid residues G133-T157. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C

Target Information

HOMER1 is a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: homer homolog 1; Homer protein homolog 1; homer, neuronal immediate early gene, 1; Homer-1; immediate early gene protein Homer1A; PSD-Zip45; scaffold protein; VASP/Ena-related gene up-regulated during seizure and LTP 1; Vesl-1

Gene Aliases: HOMER; homer-1; HOMER1; HOMER1A; HOMER1B; HOMER1C; HOMER1F; PSD-Zip45; SYN47; Ves-1; Vesl; Vesl-1; Vesl1

UniProt ID: (Human) Q86YM7, (Mouse) Q9Z2Y3, (Rat) Q9Z214

Entrez Gene ID: (Human) 9456, (Mouse) 26556, (Rat) 29546

Function(s)