Invitrogen

HOMER1 Monoclonal Antibody (OTI7G10)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (14) HOMER1 antibodies

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Datasheet

Protocols

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HOMER1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human embryonic cerebellum tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a HOMER1 monoclonal antibody (Product # MA5-27522) at a dilution of 1:500.

HOMER1 Antibody in Western Blot (WB) — Western blot was performed using Anti-HOMER1 Monoclonal Antibody (OTI7G10) (Product # MA5-27522) and a 40kDa band corresponding to HOMER1 was observed across all cell lines and tissues tested except Mouse Kidney and Rat Kidney which are reported to be negative. Whole Cell Extract-WCL (30 µg lysate) of IMR-32 (Lane 1), SK-N-AS (Lane 2), Mouse Brain (Lane 3), Mouse Skeletal Muscle (Lane 4), Mouse Kidney (Lane 5), Rat Brain (Lane 6), Rat Skeletal Muscle (Lane 7) and Rat Kidney (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

HOMER1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. IMR-32 cells were transfected with HOMER1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-HOMER1 Monoclonal Antibody (OTI7G10) (Product # MA5-27522). {KD}

Product Details

MA5-27522

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Clone	OTI7G10
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-354 of HOMER1 produced in E.coli View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2724465

Target Information

HOMER1 is a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: homer homolog 1; Homer protein homolog 1; homer, neuronal immediate early gene, 1; Homer-1; immediate early gene protein Homer1A; PSD-Zip45; scaffold protein; VASP/Ena-related gene up-regulated during seizure and LTP 1; Vesl-1

Gene Aliases: HOMER; homer-1; HOMER1; HOMER1A; HOMER1B; HOMER1C; HOMER1F; PSD-Zip45; SYN47; Ves-1; Vesl; Vesl-1; Vesl1

UniProt ID: (Human) Q86YM7, (Mouse) Q9Z2Y3, (Rat) Q9Z214

Entrez Gene ID: (Human) 9456, (Mouse) 26556, (Rat) 29546

Function(s)