Invitrogen

HOMER1 Monoclonal Antibody (OTI7G10)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (20) HOMER1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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HOMER1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded human embryonic cerebellum tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a HOMER1 monoclonal antibody (Product # MA5-27522) at a dilution of 1:500.

HOMER1 Antibody in Western Blot (WB) — Western blot was performed using Anti-HOMER1 Monoclonal Antibody (OTI7G10) (Product # MA5-27522) and a 40kDa band corresponding to HOMER1 was observed across all cell lines and tissues tested except Mouse Kidney and Rat Kidney which are reported to be negative. Whole Cell Extract-WCL (30 µg lysate) of IMR-32 (Lane 1), SK-N-AS (Lane 2), Mouse Brain (Lane 3), Mouse Skeletal Muscle (Lane 4), Mouse Kidney (Lane 5), Rat Brain (Lane 6), Rat Skeletal Muscle (Lane 7) and Rat Kidney (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

HOMER1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. IMR-32 cells were transfected with HOMER1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-HOMER1 Monoclonal Antibody (OTI7G10) (Product # MA5-27522).

Product Details

MA5-27522

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI7G10

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-354 of HOMER1 produced in E.coli

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2724465

Target Information

HOMER1 is a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: homer homolog 1; Homer protein homolog 1; homer, neuronal immediate early gene, 1; Homer-1; immediate early gene protein Homer1A; PSD-Zip45; scaffold protein; VASP/Ena-related gene up-regulated during seizure and LTP 1; Vesl-1

Gene Aliases: HOMER; homer-1; HOMER1; HOMER1A; HOMER1B; HOMER1C; HOMER1F; PSD-Zip45; SYN47; Ves-1; Vesl; Vesl-1; Vesl1

UniProt ID: (Human) Q86YM7, (Mouse) Q9Z2Y3, (Rat) Q9Z214

Entrez Gene ID: (Human) 9456, (Mouse) 26556, (Rat) 29546

Function(s)

skeletal muscle contraction G-protein coupled glutamate receptor signaling pathway protein localization to synapse behavioral response to cocaine skeletal muscle fiber development chemical homeostasis within a tissue response to calcium ion positive regulation of calcium ion transport regulation of calcium ion import regulation of store-operated calcium entry regulation of cation channel activity phospholipase C-activating G-protein coupled glutamate receptor signaling pathway chemical synaptic transmission positive regulation of signal transduction circadian rhythm response to nicotine response to cocaine

Process(es)

protein binding G-protein coupled glutamate receptor binding signaling adaptor activity ion channel binding receptor binding type 5 metabotropic glutamate receptor binding protein complex scaffold identical protein binding protein heterodimerization activity scaffold protein binding