Immunohistochemical analysis of HOXA9 using a HOXA9 polyclonal antibody (Product # PA5-23410). Formalin-fixed, paraffin-embedded human brain tissue probed with an isotype control antibody (top) and a HOXA9 polyclonal antibody (Product # PA5-23410) (bottom) at 5 ug/ml.
|Tested species reactivity||Dog, Chicken, Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (199 NWLHARSTRKKRCPYTKH 216) of HOXA9 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
HOXA9 (Homeobox protein A9) is a protein that in humans is encoded by the HOXA9 gene. In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This HOXA9 is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. HOXA9 is highly similar to the abdominal-B (Abd-B) gene of Drosophila. A specific translocation event which causes a fusion between this gene and the NUP98 gene has been associated with myeloid leukemogenesis. Transfection studies showed that HOXA9 is a strong transcriptional repressor; it suppresses the transcription of the luciferase reporter gene driven by a 124-base pair OPN promoter fragment containing both Smad3 and HOX binding sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.