Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
0.1-0.2 µg/mL | - |
ELISA (ELISA) |
0.25-1.0 µg/mL | - |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
E.coli-derived Recombinant Human Heregulinbeta-1 |
Conjugate |
Biotin |
Form |
Lyophilized |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS |
Contains |
no preservative |
Storage conditions |
-20°C |
AA Sequence of recombinant protein: SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Heregulinbeta-1. Anti-Human Heregulinbeta-1-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human Heregulin-beta1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Heregulin-beta1 (500-P288) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Heregulin-beta1.
Western Blot: To detect Human Heregulin-beta1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human Heregulin-beta1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Acetylcholine receptor-inducing activity; ARIA; Breast cancer cell differentiation factor p45; Glial growth factor; glial growth factor 2; Heregulin; heregulin, alpha (45kD, ERBB2 p185-activator); HRG; Neu differentiation factor; Neuregulin-1; Pro-neuregulin-1, membrane-bound isoform; Pro-NRG1; sensory and motor neuron derived factor; Sensory and motor neuron-derived factor
Gene Aliases: ARIA; GGF; GGF2; HGL; HRG; HRG1; HRGA; MST131; MSTP131; NDF; NRG1; NRG1-IT2; SMDF
UniProt ID: (Human) Q02297
Entrez Gene ID: (Human) 3084
Molecular Function:
growth factor
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn moreGet expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support