Western blot analysis of rat liver and fish ventricle lysates using Heat Shock Protein 70/HSC Protein 7 Monoclonal Antibody (MA1-10891). Whole tissue extracts from rat liver (20µg) and Chaenocephalus aceratus and Notothenia coriiceps ventricles (200µg) were separated on 10% SDS-PAGE gels and transferred to nitrocellulose membranes. Following transfer, membranes were blocked with 5% milk in PBST and probed with Heat Shock Protein 70/HSC Protein 7 Monoclonal Antibody (Product #MA1-10891) at 1:2000 dilution followed by donkey anti-mouse IgG secondary antibody (Product # PA1-28748) at 1:5000 dilution and a chemiluminescent substrate. Data courtesy of the Innovators Program
|Tested species reactivity||Many|
|Published species reactivity||Rabbit|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Chicken Hsp70/Hsp90 complex.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||5 ug/ml|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
MA1-10891 detects Heat Shock Protein 70/Heat Shock Cognate Protein 70 in human, mouse, rat, rabbit, monkey, hamster, guinea pig, dog, bovine, sheep, pig, chicken, yeast, fish and Xenopus samples.
MA1-10891 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, MA1-10891 is specific for the ~72 kDa Heat Shock Protein 70/Heat Shock Cognate Protein 70 protein.
MA1-10891 immunogen is chicken Hsp70/Hsp90 complex.
Members of the Hsp70 family are molecular chaperones that function in protein folding, transport, maturation and degradation by binding to nascent polypeptide chains as well as partially folded protein intermediates and preventing their aggregation and misfolding. The 70 kDa heat shock cognate protein, Hsc70, is closely related biochemically and biologically to Hsp70.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mutation of Hip's carboxy-terminal region inhibits a transitional stage of progesterone receptor assembly.
MA1-10891 was used in immunoprecipitation to investigate the effect of Hip on progesterone receptor assembly
|Prapapanich V,Chen S,Smith DF||Molecular and cellular biology (18:944)||1998|