Immunofluorescent analysis of HSD3a showing staining in the cytoplasm and nucleus of A431 cells. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a HSD3a polyclonal antibody (Product # PA5-21501) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 217 of HSD3a (Uniprot ID#P17516)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-21501 targets HSD3a in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-21501 immunogen is a recombinant fragment corresponding to a region within amino acids 1 and 217 of HSD3a (Uniprot ID#P17516).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.