|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rat / IgG1|
|Immunogen||Recombinant mouse HSF1 protein (aa1-503)|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Gel Shift (GS)||1mg/mL|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14622 targets Heat Shock Factor 1 in GS, IF, IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-14622 immunogen is recombinant mouse HSF1 protein (aa1-503).
HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents; binds to heat shock response elements (HSEs) in the upstream region of heat shock gene and activates their transcription. Some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Early-stage treatment with Withaferin A reduces levels of misfolded superoxide dismutase 1 and extends lifespan in a mouse model of amyotrophic lateral sclerosis.
MA5-14622 was used in western blot to examine the effects of Withaferin A treatment using two mouse models of amyotrophic lateral sclerosis.
|Patel P,Julien JP,Kriz J||Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics (12:217)||2015|
Two distinct disulfide bonds formed in human heat shock transcription factor 1 act in opposition to regulate its DNA binding activity.
MA5-14622 was used in western blot to study the effects on heat shock transcription factor-1 trimerization and DNA binding of two different disulfide bonds
|Lu M,Kim HE,Li CR,Kim S,Kwak IJ,Lee YJ,Kim SS,Moon JY,Kim CH,Kim DK,Kang HS,Park JS||Biochemistry (47:6007)||2008|
A novel function for HSF1-induced mitotic exit failure and genomic instability through direct interaction between HSF1 and Cdc20.
MA5-14622 was used in western blot to investigate the interaction between heat-shock factor 1 and Cdc20 and the role of heat-shock factor 1 in aneuploidy development and genomic instability
|Lee YJ,Lee HJ,Lee JS,Jeoung D,Kang CM,Bae S,Lee SJ,Kwon SH,Kang D,Lee YS||Oncogene (27:2999)||2008|
Transcription of Satellite III non-coding RNAs is a general stress response in human cells.
MA5-14622 was used in immunocytochemistry to study the transcription of Satellite III non-coding RNAs in response to stress in human cells
|Valgardsdottir R,Chiodi I,Giordano M,Rossi A,Bazzini S,Ghigna C,Riva S,Biamonti G||Nucleic acids research (36:423)||2008|
Human chromosomes 9, 12, and 15 contain the nucleation sites of stress-induced nuclear bodies.
MA5-14622 was used in immunocytochemistry to study the ability of human chromosomes 9, 12 and 15 to direct the formation of stress bodies
|Denegri M,Moralli D,Rocchi M,Biggiogera M,Raimondi E,Cobianchi F,De Carli L,Riva S,Biamonti G||Molecular biology of the cell (13:2069)||2002|
Stress-specific activation and repression of heat shock factors 1 and 2.
MA5-14622 was used in EMSA to study the differential activation and repression of HSF1 and HSF2 by different cellular stressors
|Mathew A,Mathur SK,Jolly C,Fox SG,Kim S,Morimoto RI||Molecular and cellular biology (21:7163)||2001|