|Tested species reactivity||Human, Mouse, Non-human primate|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG1|
|Immunogen||Recombinant mouse HSF2 protein (aa1-517)|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91597 detects HSF2 from mouse, non-human primate, human samples.
MA1-91597 has been successfully used in immunofluorescence, immunoprecipitation, Western blot applications.
The MA1-91597 immunogen is: Recombinant mouse HSF2 protein (aa1-517)
HSF2, as well as the related gene HSF1, encodes a protein that binds specifically to the heat-shock element and has homology to HSFs of other species. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Although the names HSF1 and HSF2 were chosen for historical reasons, these peptides should be referred to as heat-shock transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A novel function for HSF1-induced mitotic exit failure and genomic instability through direct interaction between HSF1 and Cdc20.
MA1-91597 was used in western blot to investigate the interaction between heat-shock factor 1 and Cdc20 and the role of heat-shock factor 1 in aneuploidy development and genomic instability
|Lee YJ,Lee HJ,Lee JS,Jeoung D,Kang CM,Bae S,Lee SJ,Kwon SH,Kang D,Lee YS||Oncogene (27:2999)||2008|
Stress-specific activation and repression of heat shock factors 1 and 2.
MA1-91597 was used in EMSA to study the differential activation and repression of HSF1 and HSF2 by different cellular stressors
|Mathew A,Mathur SK,Jolly C,Fox SG,Kim S,Morimoto RI||Molecular and cellular biology (21:7163)||2001|