|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Species reactivity||Guinea pig, Hamster, Mouse, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant mouse Heat Shock Protein 25.|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-25494 detects Heat Shock Protein 25 from mouse, rat, porcine, guinea pig, and hamster samples.
PA1-25494 has been successfully used in immunoprecipitation, and Western blot procedures.
The PA1-25494 immunogen is recombinant mouse Heat Shock Protein 25.
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock proteins. One class of stress proteins, termed the "small heat shock proteins" is comprised of a diverse group of proteins from ~15 to >30kDa, with a single form found in yeast and human cells and multiple related forms found in higher plants. A direct relationship between elevated strwess protein expression and neoplasia has been suggested from studies on estrogen-induced proteins in breast cancer cells. In addition, Hsp27 exhibits rapid increased phosphorylation in response to various mitogens, turmor promoter (e.g. phorbol esters) and calcium ionophores. The expression of a 24kDa protein was shown to correlate with steroid hormone receptor in cell lines, organs, and tumors. The 24kDa estrogen-induced protein was found to be expressed at high levels in carcinoma of the breast. Another study has suggested that levels of the Hsp27 correlate with endometrial adenocarcinomas. Interestingly, the regulation of human Hsp27 by estrogens and heat shock is reminiscent of the ecdysteroid and heat shock activation of the drosophila Hsp20 genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Growth-related 25 kDa protein; heat shock 10kDa protein 1-like; Heat shock 25 kDa protein; Heat shock 27 kDa protein; heat shock 27kDa protein 1; Heat shock 70 kDa protein 1-like; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 3; Heat shock 70 kDa-like protein 1; heat shock 70kDa protein 1-like; Heat shock protein 70-1l; Heat shock protein beta-1; heat shock protein cognate 70, testis; heat shock protein, 25 kDa; heat shock-related protein hsc70t; HSP 25; HSP 27; HSP25; HSP27; HSP70.3; HSPB1; inducible heat shock protein 70; p25; Spermatid-specific heat shock protein 70; truncated hsp25
Gene Aliases: 27kDa; Hsc70t; Hsp25; HSP27; Hsp70-3; HSPA1L; HSPB1; I79_007264; Msh5; SBAB-711D2.2
Molecular Function: chaperone
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