Western blot of HSP25/Heat Shock Protein 25 in PC-12 cell lysate treated with heat-shock (Lane 2), NIH-3T3 cell lysate treated with heat-shock (Lane 3) and recombinant mouse HSP25 (Lane 4) using a HSP25/Heat Shock Protein 25 polyclonal antibody (Product # PA1-25494).
|Tested species reactivity||Guinea pig, Hamster, Mouse, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant mouse Heat Shock Protein 25.|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25494 detects Heat Shock Protein 25 from mouse, rat, porcine, guinea pig, and hamster samples.
PA1-25494 has been successfully used in immunoprecipitation, and Western blot procedures.
The PA1-25494 immunogen is recombinant mouse Heat Shock Protein 25.
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock proteins. One class of stress proteins, termed the "small heat shock proteins" is comprised of a diverse group of proteins from ~15 to >30kDa, with a single form found in yeast and human cells and multiple related forms found in higher plants. A direct relationship between elevated strwess protein expression and neoplasia has been suggested from studies on estrogen-induced proteins in breast cancer cells. In addition, Hsp27 exhibits rapid increased phosphorylation in response to various mitogens, turmor promoter (e.g. phorbol esters) and calcium ionophores. The expression of a 24kDa protein was shown to correlate with steroid hormone receptor in cell lines, organs, and tumors. The 24kDa estrogen-induced protein was found to be expressed at high levels in carcinoma of the breast. Another study has suggested that levels of the Hsp27 correlate with endometrial adenocarcinomas. Interestingly, the regulation of human Hsp27 by estrogens and heat shock is reminiscent of the ecdysteroid and heat shock activation of the drosophila Hsp20 genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.