MA3-015 detects heat shock protein 27 kDa (HSP27) from human, rat, mouse, monkey, and canine samples.
MA3-015 has been successfully used in Western blot, immunohistochemistry (paraffin), immunoprecipitation, immunofluorescence, immunocytochemistry and ELISA procedures. By Western blot, this antibody detects an ~24 kDa protein representing HSP27 in MCF-7 cell extract. Immunohistochemical staining of human cervix with MA3-015 results in intense cytoplasmic staining of the epithelium within the parabasal cell layer. Western blot detection of HSP27 in canine heart samples is weak and may require optimization.
The MA3-015 antigen is partially purified HSP27 derived from MCF-7 cytosol. The mouse HSP27 sequence is strongly conserved within the human sequence, so cross-reactivity with mouse HSP27 is expected.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. HSP27 also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that HSP27 may be a useful marker in classifying various hormone sensitive tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 28 kDa heat shock protein; epididymis secretory protein Li 102; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; heat shock protein 27 kDa beta-1; Heat shock protein beta-1; HSP 27; HSP25; HSP27; HSPB1; SRP27; Stress-responsive protein 27
Gene Aliases: CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; SRP27
Molecular Function: chaperone