|ELISA (ELISA)||1-10 µg/ml|
|Immunohistochemistry (IHC)||1-10 µg/ml|
|Immunoprecipitation (IP)||2 µg|
|Western Blot (WB)||1-10 µg/ml|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgM|
|Immunogen||Human placental HSP60.|
|Storage buffer||1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-013 detects HSP60 from human, monkey, mouse, and rat samples. This antibody displays no other protein reactivity.
MA3-013 has been successfully used in Western blot, immunoprecipitation, immunohistochemistry, immunofluorescence, and ELISA procedures. By Western blot, this antibody detects a single 58 kDa protein representing recombinant human HSP60.
The MA3-013 antigen is human placental HSP60. Epitope mapping studies using human HSP60 deletion mutants suggest that this antibody binds between amino acids 211-288.
Reconstitute with PBS.
Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. HSP60, an ~60 kDa protein, is the human homologue of HSP65 (a major antigenic mycobacterial protein) and E. coli GroEL. HSP60, also referred to as chaperonin-60, P1 and mitonin, has been classified as a member of a family of proteins termed chaperonins which act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly. HSP60 is a constitutively expressed mitochondrial protein that shares many characteristics with HSP70 including high abundance, induction by environmental stress, ATPase activity and a critical role in protein assembly and folding. However, in contrast with HSP70, HSP60 functions as a 14 subunit oligomer which is thought to interact with only 1 or 2 target polypeptides at one time.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 60 kDa chaperonin; 60 kDa heat shock protein; 60 kDa heat shock protein, mitochondrial; Chaperonin 60; CPN60; GROEL; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1; heat shock 60kDa protein 1 (chaperonin); Heat shock protein 60; heat shock protein 60 (liver); heat shock protein 65; heat shock protein, 60 kDa; HLD4; HSP-60; HSP-65; HSP65; HuCHA60; mitochondrial; Mitochondrial matrix protein P1; Mytonin Chaperone 60; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; SPG13
Gene Aliases: 60kDa; CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HSPD1; Hspd1-30p; HuCHA60; SPG13
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.