|Tested species reactivity||Bacteria|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant Chlamydia trachomatis HSP60.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:5,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA3-022 detects heat shock protein 60 kDa (HSP60) from bacteria. This antibody detects HSP60 from all three species of Chlamydia, Escherichia coli (GroEL), Salmonella typhimurium, Neisseria gonorrhoeae and Coxiella burnetti. MA3-022 does not cross react with HeLa cell HSP60.
MA3-022 has been successfully used in Western blot procedures. By Western blot, this antibody detects a single ~60 kDa protein representing HSP60 from C. trachomatis lysate.
The MA3-022 immunogen is recombinant Chlamydia trachomatis HSP60. Epitope mapping studies suggest that this antibody binds to a linear sequence of 6 amino acids of Chlamydia HSP60 amino acids 117-122.
Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. HSP60, an ~60 kDa protein, is the human homologue of HSP65 (a major antigenic mycobacterial protein) and E. coli GroEL. HSP60, also referred to as chaperonin-60, P1 and mitonin, has been classified as a member of a family of proteins termed chaperonins which act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly. HSP60 is a constitutively expressed mitochondrial protein that shares many characteristics with HSP70 including high abundance, induction by environmental stress, ATPase activity and a critical role in protein assembly and folding. However, in contrast with HSP70, HSP60 functions as a 14 subunit oligomer which is thought to interact with only 1 or 2 target polypeptides at one time.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Monoclonal antibodies define genus-specific, species-specific, and cross-reactive epitopes of the chlamydial 60-kilodalton heat shock protein (hsp60): specific immunodetection and purification of chlamydial hsp60.
MA3-022 was used in western blot and ELISA to produce chlamydial hsp6O antibodies and characterize their respective specifity
|Yuan Y,Lyng K,Zhang YX,Rockey DD,Morrison RP||Infection and immunity (60:2288)||1992|