Western blot analysis of Hsp60 in the 1) absence and 2) presence of the immunizing peptide in HeLa cell lysate and the 3) absence and 4) presence of immunizing peptide in Jurkat cell lysate using a HSP60/Heat Shock Protein 60 polyclonal antibody (Product # PA1-41662) at a dilution of 0.2 ug/ml.
|Tested species reactivity||Bovine, Dog, Chicken, Human, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 350-400 of human Hsp60.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.2-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: HeLa, Jurkat cell lysate, Hela whole cell lysate, antigen standard for HSPD1 (transient overexpression lysate), Jurkat whole cell lysate.
HSP60, a 60KDa member of Chaperonin family, is a mitochondrial protein that participates in protein folding, transport and secretion. HSP60 is a multi-functional protein whose roles include accelerating the maturation of pro-caspase 3 during apoptosis, LPS-binding, acting as a ligand for innate immune system and also binding to the surface of innate immune cells and stimulating proinflammatory reactivity. It induces the expression of IL-12 and IL-15 and also induces release of TNF-alpha, NO and IL-6. Reports suggest interaction of Hsp60 with integrin molecules during metastasis. Mutations in Hsp60 gene cause autosomal recessive spastic paraplegia 13. Hsp60 has been found to be involved in development of auto-immune disorders such as Rheumatoid arthritis, insulin-dependent diabetes mellitus, systemic sclerosis and atherosclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.