Immunofluorescent analysis of HeLa cells using a HSP60/Heat Shock Protein 60 polyclonal antibody (Product # PA5-12484). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a HSP60/Heat Shock Protein 60 polyclonal antibody (Product # PA5-12484) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 187-215 amino acids from the central region of human HSPD1|
|Purification||Size-exclusion - Dialysis, Ammonium sulfate precipitation|
|Contains||0.09% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
This antibody is predicted to react with bovine, chicken, hamster, mouse and rat based on sequence homology.
HSPD1 is a member of the chaperonin family. This protein may function as a signaling molecule in the innate immune system. The protein is essential for the folding and assembly of newly imported proteins in the mitochondria. The protein is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Identification of LACTB2, a metallo-ß-lactamase protein, as a human mitochondrial endoribonuclease.
PA5-12484 was used in western blot to discover that LACTB2 is an endoribonuclease that processes mitochondrial RNA
|Levy S,Allerston CK,Liveanu V,Habib MR,Gileadi O,Schuster G||Nucleic acids research (44:1813)||2016|