Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescent analysis of HeLa cells using a HSP60/Heat Shock Protein 60 polyclonal antibody (Product # PA5-12484). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a HSP60/Heat Shock Protein 60 polyclonal antibody (Product # PA5-12484) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 187-215 amino acids from the central region of human HSPD1|
|Purification||Size-exclusion - Dialysis, Ammonium sulfate precipitation|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, chicken, hamster, mouse and rat based on sequence homology.
HSPD1 is a member of the chaperonin family. This protein may function as a signaling molecule in the innate immune system. The protein is essential for the folding and assembly of newly imported proteins in the mitochondria. The protein is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
60 kDa chaperonin; 60 kDa heat shock protein; chaperonin 60; GROEL; heat shock 60kDa protein 1 (chaperonin); heat shock protein 65; HLD4; HSP-60; HSP65; mitochondrial; mitochondrial matrix protein P1; Mytonin Chaperone 60; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; SPG13
CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HSPD1; HuCHA60; SPG13