Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
|Tested species reactivity||Eubacteria|
|Published species reactivity||Eubacteria, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant Chlamydia trachomatis HSP60 protein.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-023 detects heat shock protein 60 kDa (HSP60) from bacteria. This antibody detects all three species of Chlamydia HSP60 with minimal cross-reactivity with Borrelia burgdorferi HSP60. MA3-023 does not react with E. coli GroEL.
MA3-023 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a single ~60 kDa band representing HSP60 from C. trachomatis lysate.
The MA3-023 immunogen is recombinant Chlamydia trachomatis HSP60. Epitope mapping studies suggest that this antibody binds to a linear sequence of Chlamydia HSP60 amino acids 517-522.
Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. HSP60, an ~60 kDa protein, is the human homologue of HSP65 (a major antigenic mycobacterial protein) and E. coli GroEL. HSP60, also referred to as chaperonin-60, P1 & mitonin, has been classified as a member of a family of proteins termed chaperonins which act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly. HSP60 is a constitutively expressed mitochondrial protein that shares many characteristics with HSP70 including high abundance, induction by environmental stress, ATPase activity and a critical role in protein assembly and folding. However, in contrast with HSP70, HSP60 functions as a 14 subunit oligomer which is thought to interact with only 1 or 2 target polypeptides at one time.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The intracellular bacteria Chlamydia hijack peroxisomes and utilize their enzymatic capacity to produce bacteria-specific phospholipids.
MA3-023 was used in immunocytochemistry to study the synthesis of bacteria-specific phospholipids in peroxisomes of cells infected with the intracellular bacterial pathogen Chlamydia
|Boncompain G,Müller C,Meas-Yedid V,Schmitt-Kopplin P,Lazarow PB,Subtil A||PloS one (9:null)||2014|
Chlamydia pneumoniae infection in circulating human monocytes is refractory to antibiotic treatment.
MA3-023 was used in immunocytochemistry to investigate the influence of rifampin and azithromycin on chlamydial growth and activity within monocytes
|Gieffers J,Füllgraf H,Jahn J,Klinger M,Dalhoff K,Katus HA,Solbach W,Maass M||Circulation (103:351)||2001|
Morphologic and antigenic characterization of interferon gamma-mediated persistent Chlamydia trachomatis infection in vitro.
MA3-023 was used in immunocytochemistry to investigate the effect of gamma interferon on Chlamydia trachomatis infection
|Beatty WL,Byrne GI,Morrison RP||Proceedings of the National Academy of Sciences of the United States of America (90:3998)||1993|
Association of carotid plaque Lp-PLA(2) with macrophages and Chlamydia pneumoniae infection among patients at risk for stroke.
MA3-023 was used in immunohistochemistry to investigate the interplay among lipoprotein-associated phospholipase A2, macrophages, and Chlamydia pneumoniae infection in atherosclerosis
|Atik B,Johnston SC,Dean D||PloS one (5:null)||2010|
Matrix metalloproteinase-9 expression is associated with the presence of Chlamydia pneumoniae in human coronary atherosclerotic plaques.
MA3-023 was used in immunohistochemistry to study the relationship between MMP-9 expression and the presence of Chlamydia pneumoniae in atherosclerotic plaques
|Arno G,Kaski JC,Smith DA,Akiyu JP,Hughes SE,Baboonian C||Heart (British Cardiac Society) (91:521)||2005|
Chlamydia pneumoniae in abdominal aortic aneurysms: abundance of membrane components in the absence of heat shock protein 60 and DNA.
MA3-023 was used in immunohistochemistry to detect chlamydia pneumoniae in abdominal aortic aneurysms
|Meijer A,van Der Vliet JA,Roholl PJ,Gielis-Proper SK,de Vries A,Ossewaarde JM||Arteriosclerosis, thrombosis, and vascular biology (19:2680)||1999|
Chlamydophila pneumoniae infection leads to smooth muscle cell proliferation and thickening in the coronary artery without contributions from a host immune response.
MA3-023 was used in western blot to study the induction of smooth muscle cell proliferation and vascular thickening during Chlamydophila pneumoniae infection
|Deniset JF,Cheung PK,Dibrov E,Lee K,Steigerwald S,Pierce GN||The American journal of pathology (176:1028)||2010|
Tryptophan depletion as a mechanism of gamma interferon-mediated chlamydial persistence.
MA3-023 was used in western blot to investigate the role of gamma interferon in chlamydial persistence
|Beatty WL,Belanger TA,Desai AA,Morrison RP,Byrne GI||Infection and immunity (62:3705)||1994|