|Tested species reactivity||Bacteria|
|Published species reactivity||Bacteria, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant Chlamydia trachomatis HSP60 protein.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-023 detects heat shock protein 60 kDa (HSP60) from bacteria. This antibody detects all three species of Chlamydia HSP60 with minimal cross-reactivity with Borrelia burgdorferi HSP60. MA3-023 does not react with E. coli GroEL.
MA3-023 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a single ~60 kDa band representing HSP60 from C. trachomatis lysate.
The MA3-023 immunogen is recombinant Chlamydia trachomatis HSP60. Epitope mapping studies suggest that this antibody binds to a linear sequence of Chlamydia HSP60 amino acids 517-522.
Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. HSP60, an ~60 kDa protein, is the human homologue of HSP65 (a major antigenic mycobacterial protein) and E. coli GroEL. HSP60, also referred to as chaperonin-60, P1 and mitonin, has been classified as a member of a family of proteins termed chaperonins which act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly. HSP60 is a constitutively expressed mitochondrial protein that shares many characteristics with HSP70 including high abundance, induction by environmental stress, ATPase activity and a critical role in protein assembly and folding. However, in contrast with HSP70, HSP60 functions as a 14 subunit oligomer which is thought to interact with only 1 or 2 target polypeptides at one time.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Chlamydia Infection Across Host Species Boundaries Promotes Distinct Sets of Transcribed Anti-Apoptotic Factors.
MA3-023 was used in immunocytochemistry to research promotion of distinct sets of transcribed anti-apoptotic factors due to chlamydia infection across host species boundaries
|Messinger JE,Nelton E,Feeney C,Gondek DC||Frontiers in cellular and infection microbiology (5:null)||2016|
The intracellular bacteria Chlamydia hijack peroxisomes and utilize their enzymatic capacity to produce bacteria-specific phospholipids.
MA3-023 was used in immunocytochemistry to study the synthesis of bacteria-specific phospholipids in peroxisomes of cells infected with the intracellular bacterial pathogen Chlamydia
|Boncompain G,Müller C,Meas-Yedid V,Schmitt-Kopplin P,Lazarow PB,Subtil A||PloS one (9:null)||2014|
Chlamydia pneumoniae infection in circulating human monocytes is refractory to antibiotic treatment.
MA3-023 was used in immunocytochemistry to investigate the influence of rifampin and azithromycin on chlamydial growth and activity within monocytes
|Gieffers J,Füllgraf H,Jahn J,Klinger M,Dalhoff K,Katus HA,Solbach W,Maass M||Circulation (103:351)||2001|
Morphologic and antigenic characterization of interferon gamma-mediated persistent Chlamydia trachomatis infection in vitro.
MA3-023 was used in immunocytochemistry to investigate the effect of gamma interferon on Chlamydia trachomatis infection
|Beatty WL,Byrne GI,Morrison RP||Proceedings of the National Academy of Sciences of the United States of America (90:3998)||1993|
Association of carotid plaque Lp-PLA(2) with macrophages and Chlamydia pneumoniae infection among patients at risk for stroke.
MA3-023 was used in immunohistochemistry to investigate the interplay among lipoprotein-associated phospholipase A2, macrophages, and Chlamydia pneumoniae infection in atherosclerosis
|Atik B,Johnston SC,Dean D||PloS one (5:null)||2010|
Matrix metalloproteinase-9 expression is associated with the presence of Chlamydia pneumoniae in human coronary atherosclerotic plaques.
MA3-023 was used in immunohistochemistry to study the relationship between MMP-9 expression and the presence of Chlamydia pneumoniae in atherosclerotic plaques
|Arno G,Kaski JC,Smith DA,Akiyu JP,Hughes SE,Baboonian C||Heart (British Cardiac Society) (91:521)||2005|
Chlamydia pneumoniae in abdominal aortic aneurysms: abundance of membrane components in the absence of heat shock protein 60 and DNA.
MA3-023 was used in immunohistochemistry to detect chlamydia pneumoniae in abdominal aortic aneurysms
|Meijer A,van Der Vliet JA,Roholl PJ,Gielis-Proper SK,de Vries A,Ossewaarde JM||Arteriosclerosis, thrombosis, and vascular biology (19:2680)||1999|
Chlamydophila pneumoniae infection leads to smooth muscle cell proliferation and thickening in the coronary artery without contributions from a host immune response.
MA3-023 was used in western blot to study the induction of smooth muscle cell proliferation and vascular thickening during Chlamydophila pneumoniae infection
|Deniset JF,Cheung PK,Dibrov E,Lee K,Steigerwald S,Pierce GN||The American journal of pathology (176:1028)||2010|
Tryptophan depletion as a mechanism of gamma interferon-mediated chlamydial persistence.
MA3-023 was used in western blot to investigate the role of gamma interferon in chlamydial persistence
|Beatty WL,Belanger TA,Desai AA,Morrison RP,Byrne GI||Infection and immunity (62:3705)||1994|