|Flow Cytometry (Flow)||1:100 - 1:200|
|Inhibition Assays (IA)||Assay Dependent|
|Immunohistochemistry (IHC)||1:20 - 50|
|Western Blot (WB)||1:1000 - 1:5000|
|Western Blot (WB)||See 10 publications below|
|Neutralization (Neu)||See 1 publications below|
|Cytotoxicity Assay (CT)||See 1 publications below|
|Flow Cytometry (Flow)||See 2 publications below|
|Immunomicroscopy (IM)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Non-human primate Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant HSP70 over expressed in E. coli.|
|Storage buffer||ascites diluted in PBS|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-009 detects heat shock protein 70 kDa (HSP70) from mouse and human tissues. This antibody specifically detects HSP70 and does not cross-react with other members of the HSP70 family.
MA3-009 has been successfully used in Western blot, IF, blocking, FACS, inhibition assay, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 70 kDa protein representing HSP70 from K562 cell extract.
The MA3-009 antigen is recombinant human HSP70 over-expressed in E. coli. Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 504-617 of human HSP70, a region that has been shown to be involved in stress-induced nucleolar localization.
Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 68 kDa heat shock protein; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; Heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock protein, 70 kDa 3; heat shock-induced protein; heat shock-induced protein, Hspa70; Hsp68; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-2; HSP70.1/HSP70.2; HSP70.3; HSP70I; HSP72; HSPA1; inducible heat shock protein 70
Gene Aliases: HEL-S-103; hsp68; HSP70-1; HSP70-1A; Hsp70-3; HSP70.1; Hsp70.3; Hsp70A1; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B; HSX70
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.