Immunofluorescent analysis of Heat Shock Protein 70 (HSP70) (green) in HeLa and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a HSP70 Monoclonal Antibody (Product # MA3-007), at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Amphibian, Avian, Fruit fly, Fish, Flatworm, Human, Mouse, Rat, Yeast|
|Published species reactivity||Rabbit, Yeast, Rat, Fruit fly, Mollusc, Amphibian, Bovine, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant HSP70 over expressed in E. coli.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Gel Shift (GS)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:250 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 25 publications below|
|Immunohistochemistry (IHC)||See 4 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Gel Shift (GS)||See 1 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
MA3-007 detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70, GRP78, and following heat shock, HSP72 from yeast, rat, Drosophila, fish, mouse, avian, amphibian and human samples.
MA3-007 has been successfully used in Western blot, immunofluorescence, gel shift, immunohistochemistry (frozen), and immunoprecipitation procedures. By Western blot, this antibody detects proteins from ~70 kDa to ~78 kDa representing different members of the HSP70 family. 2-dimensional gel electrophoresis is required to resolve the heat induced form of these proteins from their constitutively expressed counterparts. Immunofluorescence staining of HSP70 in heat shocked HeLa cells with MA3-007 results in cytoplasmic staining.
The MA3-007 antigen is recombinant human HSP70 over-expressed in E. coli. Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 122-264 of human HSP70, a region that has been shown to be involved in ATP binding. This is the first monoclonal antibody reported to react with: 1) the ATP binding region of HSP70, 2) an epitope in the amino terminus of HSP70.
Heat shock proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP70, HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Intermittent hypoxia leads to functional reorganization of mitochondria and affects cellular bioenergetics in marine molluscs.
MA3-007 was used in western blot to determine the affect of cellular bioenergetics in marine molluscs and functional reorganization of mitochondria by intermittent hypoxia
|Ivanina AV,Nesmelova I,Leamy L,Sokolov EP,Sokolova IM||The Journal of experimental biology (219:1659)||2016|
|Not Applicable||Not Cited||
Exosomes Derived from Squamous Head and Neck Cancer Promote Cell Survival after Ionizing Radiation.
MA3-007 was used in western blot to calculate how ionizing radiation can promote cell survival in exosomes derived from squamous head and neck cancer
|Mutschelknaus L,Peters C,Winkler K,Yentrapalli R,Heider T,Atkinson MJ,Moertl S||PloS one (11:null)||2016|
Integrative proteomics and targeted transcriptomics analyses in cardiac endothelial cells unravel mechanisms of long-term radiation-induced vascular dysfunction.
MA3-007 was used in western blot to elucidate the factors that mediate radiation-induced endothelial dysfunction.
|Azimzadeh O,Sievert W,Sarioglu H,Merl-Pham J,Yentrapalli R,Bakshi MV,Janik D,Ueffing M,Atkinson MJ,Multhoff G,Tapio S||Journal of proteome research (14:1203)||2015|
|Not Applicable||Not Cited||
Temperature-dependent toxicities of nano zinc oxide to marine diatom, amphipod and fish in relation to its aggregation size and ion dissolution.
MA3-007 was used in western blot to study the toxicity of nano zinc oxide particles to selected marine organisms
|Wong SW,Leung KM||Nanotoxicology (8 Suppl 1:24)||2014|
Charcot-Marie-Tooth disease CMT4A: GDAP1 increases cellular glutathione and the mitochondrial membrane potential.
MA3-007 was used in western blot to study the effect of a GDAP1 mutation on cellular glutathione and mitochondrial membrane potential
|Noack R,Frede S,Albrecht P,Henke N,Pfeiffer A,Knoll K,Dehmel T,Meyer Zu Hörste G,Stettner M,Kieseier BC,Summer H,Golz S,Kochanski A,Wiedau-Pazos M,Arnold S,Lewerenz J,Methner A||Human molecular genetics (21:150)||2012|
Thermal adaptation in the intertidal snail Echinolittorina malaccana contradicts current theory by revealing the crucial roles of resting metabolism.
MA3-007 was used in western blot to investigate the involvement of resting metabolism in thermal adaptation theory
|Marshall DJ,Dong YW,McQuaid CD,Williams GA||The Journal of experimental biology (214:3649)||2011|
Shotgun proteomics analysis reveals new unsuspected molecular effectors of nitrogen-containing bisphosphonates in osteocytes.
MA3-007 was used in western blot to investigate the effect of nitrogen-containing bisphosphonate treatment in murine osteocytes
|Bivi N,Picotti P,Müller LN,Romanello M,Moro L,Quadrifoglio F,Tell G||Journal of proteomics (74:1113)||2011|
Thermal constraints for range expansion of the invasive green mussel, Perna viridis, in the southeastern United States.
MA3-007 was used in western blot to investigate the effect of temperature on the expansion of an invasive mussel species
|Urian AG,Hatle JD,Gilg MR||Journal of experimental zoology. Part A, Ecological genetics and physiology (315:12)||2011|
Accumulation of neoplastic traits prior to spontaneous in vitro transformation of rat cholangiocytes determines susceptibility to activated ErbB-2/Neu.
MA3-007 was used in western blot to study the in vitro transformation of rat cholangiocytes
|Rozich RA,Mills DR,Brilliant KE,Callanan HM,Yang D,Tantravahi U,Hixson DC||Experimental and molecular pathology (89:248)||2010|
|Fruit fly||Not Cited||
The SR protein B52/SRp55 is required for DNA topoisomerase I recruitment to chromatin, mRNA release and transcription shutdown.
MA3-007 was used in western blot to study the role of SR protein B52/SRp55 in the recruitment of DNA topoisomerase I to genomic activities
|Juge F,Fernando C,Fic W,Tazi J||PLoS genetics (6:null)||2010|
Elevated expression of Foxp3 in tumor-infiltrating Treg cells suppresses T-cell proliferation and contributes to gastric cancer progression in a COX-2-dependent manner.
MA3-007 was used in western blot to study the Foxp3 expression in tumor-infiltrating Treg cells and its effect on T-cell proliferation and gastric cancer progression
|Yuan XL,Chen L,Li MX,Dong P,Xue J,Wang J,Zhang TT,Wang XA,Zhang FM,Ge HL,Shen LS,Xu D||Clinical immunology (Orlando, Fla.) (134:277)||2010|
Effect of progerin on the accumulation of oxidized proteins in fibroblasts from Hutchinson Gilford progeria patients.
MA3-007 was used in western blot to investigate the influence of progerin on oxidative stress responses in normal fibrblasts and those from Hutchinson Gilford progeria patients
|Viteri G,Chung YW,Stadtman ER||Mechanisms of ageing and development (131:2)||2010|
Surface expression of a C-terminal alpha-helix region in heat shock protein 72 on murine LL/2 lung carcinoma can be recognized by innate immune sentinels.
MA3-007 was used in western blot to study the induction of heat shock protein 72 expression in murine LL/2 lung carcinoma
|Tani F,Ohno M,Furukawa Y,Sakamoto M,Masuda S,Kitabatake N||Molecular immunology (46:1326)||2009|
Effects of elevated temperature and cadmium exposure on stress protein response in eastern oysters Crassostrea virginica (Gmelin).
MA3-007 was used in western blot to investigate the influence of temperature and cadmium on protein expression in oysters
|Ivanina AV,Taylor C,Sokolova IM||Aquatic toxicology (Amsterdam, Netherlands) (91:245)||2009|
Oxidative stress and expression of chaperones in aging mollusks.
MA3-007 was used in western blot to investigate the age-dependent oxidative stress and heat shock response in mollusks
|Ivanina AV,Sokolova IM,Sukhotin AA||Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology (150:53)||2008|
Effects of cadmium on cellular protein and glutathione synthesis and expression of stress proteins in eastern oysters, Crassostrea virginica Gmelin.
MA3-007 was used in western blot to investigate the effects of cadmium exposure on expression of heat shock proteins (HSPs) in eastern oysters
|Ivanina AV,Cherkasov AS,Sokolova IM||The Journal of experimental biology (211:577)||2008|
Heat shock-mediated thermoprotection of larval locomotion compromised by ubiquitous overexpression of Hsp70 in Drosophila melanogaster.
MA3-007 was used in western blot to investigate the changes of endoplasmic reticulum chaperone complexes, redox state, and impaired protein disulfide reductase activity in misfolding alpha1-antitrypsin transgenic mice
|Klose MK,Chu D,Xiao C,Seroude L,Robertson RM||Journal of neurophysiology (94:3563)||2005|
Synaptic thermoprotection in a desert-dwelling Drosophila species.
MA3-007 was used in western blot to investigate the involvement of heat shock proteins in synaptic thermoprotection
|Newman AE,Xiao C,Robertson RM||Journal of neurobiology (64:170)||2005|
Reversible inhibition of Hsp70 chaperone function by Scythe and Reaper.
MA3-007 was used in western blot to study the role of Scythe and Reaper in Hsp70 chaperone function.
|Thress K,Song J,Morimoto RI,Kornbluth S||The EMBO journal (20:1033)||2001|
Effect of ischemic pretreatment on heat shock protein 72, neurologic outcome, and histopathologic outcome in a rabbit model of spinal cord ischemia.
MA3-007 was used in western blot to investigate the effect of ischemic pretreatment for HSP72 expression and neural damage in rabbit
|de Haan P,Vanicky I,Jacobs MJ,Bakker O,Lips J,Meylaerts SA,Kalkman CJ||The Journal of thoracic and cardiovascular surgery (120:513)||2000|
Agonist-free transformation of the glucocorticoid receptor in human B-lymphoma cells.
MA3-007 was used in western blot to study the role of cell-cell interactions in the ligand-independent nuclear translocation of the glucocorticoid receptor
|van den Berg JD,Smets LA,van Rooij H||The Journal of steroid biochemistry and molecular biology (57:239)||1996|
|Fruit fly||Not Cited||
Muscle-specific expression of Drosophila hsp70 in response to aging and oxidative stress.
MA3-007 was used in western blot to study the effect of aging and oxidative stress on the expression of HSP70 in Drosophila muscle
|Wheeler JC,Bieschke ET,Tower J||Proceedings of the National Academy of Sciences of the United States of America (92:10408)||1995|
hsc70 moderates the heat shock (stress) response in Xenopus laevis oocytes and binds to denatured protein inducers.
MA3-007 was used in western blot to study how hsc70 reduces the stress response in Xenopus oocytes
|Mifflin LC,Cohen RE||The Journal of biological chemistry (269:15718)||1994|
A constitutive form of heat-shock protein 70 is located in the outer membranes of mitochondria from rat liver.
MA3-007 was used in western blot to study the role of heat-shock protein 70 in the outer membranes of mitochondria from rat liver
|Lithgow T,Ryan M,Anderson RL,Høj PB,Hoogenraad NJ||FEBS letters (332:277)||1993|
Candidate vaccine antigens identified by antibodies from mice vaccinated with 15- or 50-kilorad-irradiated cercariae of Schistosoma mansoni.
MA3-007 was used in western blot to evaluate the vaccine antigen candidates for Schistosoma mansoni
|Richter D,Harn DA||Infection and immunity (61:146)||1993|
Characterization of the properties of a novel mutation in VAPB in familial amyotrophic lateral sclerosis.
MA3-007 was used in immunohistochemistry to investigate the role of a vesicle-associated membrane protein-associated protein B mutation in the pathology of familial amyotrophic lateral sclerosis
|Chen HJ,Anagnostou G,Chai A,Withers J,Morris A,Adhikaree J,Pennetta G,de Belleroche JS||The Journal of biological chemistry (285:40266)||2010|
Neurodegeneration by polyglutamine Atrophin is not rescued by induction of autophagy.
MA3-007 was used in immunohistochemistry to investigate the effect of autophagy in the pathogenesis of dentatorubral-pallidoluysian Atrophy
|Nisoli I,Chauvin JP,Napoletano F,Calamita P,Zanin V,Fanto M,Charroux B||Cell death and differentiation (17:1577)||2010|
A Drosophila model of oculopharyngeal muscular dystrophy reveals intrinsic toxicity of PABPN1.
MA3-007 was used in immunohistochemistry to study the intrinsic toxicity of PABPN1 in a Drosophila model of oculopharyngeal muscular dystrophy
|Chartier A,Benoit B,Simonelig M||The EMBO journal (25:2253)||2006|
Oolemmal proteomics--identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane.
MA3-007 was used in immunohistochemistry to identify highly abundant heat shock proteins and chaperone proteins in mature mouse egg proteome.
|Calvert ME,Digilio LC,Herr JC,Coonrod SA||Reproductive biology and endocrinology : RB and E (1:null)||2003|
Neuroprotection resulting from insufficiency of RANBP2 is associated with the modulation of protein and lipid homeostasis of functionally diverse but linked pathways in response to oxidative stress.
MA3-007 was used in immunocytochemistry to investigate the neuroprotective effect of RANBP2 insufficiency against light damage
|Cho KI,Yi H,Tserentsoodol N,Searle K,Ferreira PA||Disease models and mechanisms (3:595)||2010|
Novel fluorescent cycloheximide derivatives for the imaging of protein synthesis.
MA3-007 was used in immunocytochemistry to evaluate the novel fluorescent derivatives of protein synthesis studcycloheximide as reporters for cellular function study
|Paoletti F,Ainger K,Donati I,Scardigli R,Vetere A,Cattaneo A,Campa C||Biochemical and biophysical research communications (396:258)||2010|
Similarities between the DNA replication initiators of Gram-negative bacteria plasmids (RepA) and eukaryotes (Orc4p)/archaea (Cdc6p).
MA3-007 was used in immunoprecipitation to investigate the evolutionary conservation of the DNA replication initiators between Gram-negative bacteria plasmids RepA and eukaryotes Orc4p.
|Giraldo R,Diaz-Orejas R||Proceedings of the National Academy of Sciences of the United States of America (98:4938)||2001|
Complex regulation of the yeast heat shock transcription factor.
MA3-007 was used in EMSA to investigate the regulatory roles of yeast heat shock transcription factor.
|Bonner JJ,Carlson T,Fackenthal DL,Paddock D,Storey K,Lea K||Molecular biology of the cell (11:1739)||2000|
Hsp70 prevents activation of stress kinases. A novel pathway of cellular thermotolerance.
MA3-007 was used in blocking/activating experiment to investigate the role of Hsp70 in modulation of stress kinase activity
|Gabai VL,Meriin AB,Mosser DD,Caron AW,Rits S,Shifrin VI,Sherman MY||The Journal of biological chemistry (272:18033)||1997|