Western blot analysis of HSP70/Heat Shock Protein 70 in Bovine brain extract. Samples were probed with a HSP70/Heat Shock Protein 70 monoclonal antibody (Product # MA1-91159) at a dilution of 1:10,000. The antibody was developed with AlkPhos APA mouse Fab ads HIgG and a NBTBCIP substrate.
|Tested species reactivity||Bovine, C. elegans, Chicken, Fruit fly, Guinea pig, Hamster, Human, Plant, Pig, Rabbit, Rat|
|Published species reactivity||Bovine, Chicken, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full length native protein (purified) (Bovine brain).|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Dot blot (DB)||1:5000|
|ELISA (ELISA)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91159 detects Hsp70 from porcine, chicken, rabbit, rat, Drosophila melanogaster, guinea pig, hamster, plant, C.elegans, and bovine samples.
MA1-91159 has been successfully used in ELISA, immunofluorescence, immunomicroscopy, dot blot, Western blot, immunohistochemistry (frozen tissue) applications. In Western Blotting the antibody localizes both the constitutive (HSP73) and the inducible (HSP72) forms of HSP70.
The MA1-91159 immunogen is: Full length native protein (purified) (Bovine brain).
Store at 4°C for short term. For extended storage, aliquot and store at -20°C or below. Avoid Freeze/Thaw Cycles.
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MICU1 regulation of mitochondrial Ca(2+) uptake dictates survival and tissue regeneration.
MA1-91159 was used in western blot to characterize how mitochondrial Ca(21) uptake through MICU1 regulation dictates tissue regeneration and survival
|Antony AN,Paillard M,Moffat C,Juskeviciute E,Correnti J,Bolon B,Rubin E,Csordás G,Seifert EL,Hoek JB,Hajnóczky G||Nature communications (7:null)||2016|
The effect of heat stress on gene expression and synthesis of heat-shock and milk proteins in bovine mammary epithelial cells.
MA1-91159 was used in immunocytochemistry to assess the effects of heat stress on bovine mammary epithelial cells
|Hu H,Zhang Y,Zheng N,Cheng J,Wang J||Animal science journal = Nihon chikusan Gakkaiho (87:84)||2016|
Chronic Mild Cold Conditioning Modulates the Expression of Hypothalamic Neuropeptide and Intermediary Metabolic-Related Genes and Improves Growth Performances in Young Chicks.
MA1-91159 was used in western blot to examine the effect of chronic mild cold conditioning on birds
|Nguyen P,Greene E,Ishola P,Huff G,Donoghue A,Bottje W,Dridi S||PloS one (10:null)||2015|