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Western blot analysis of HSP70/Heat Shock Protein 70 in Bovine brain extract. Samples were probed with a HSP70/Heat Shock Protein 70 monoclonal antibody (Product # MA1-91159) at a dilution of 1:10,000. The antibody was developed with AlkPhos APA mouse Fab ads HIgG and a NBTBCIP substrate.
|Tested species reactivity||Bovine, Nematode, Chicken, Fruit fly, Guinea pig, Hamster, Human, Plant, Pig, Rabbit, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full length native protein (purified) (Bovine brain).|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Dot blot (DB)||1:5000|
|ELISA (ELISA)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91159 detects Hsp70 from porcine, chicken, rabbit, rat, Drosophila melanogaster, guinea pig, hamster, plant, C.elegans, and bovine samples.
MA1-91159 has been successfully used in ELISA, immunofluorescence, immunomicroscopy, dot blot, Western blot, immunohistochemistry (frozen tissue) applications. In Western Blotting the antibody localizes both the constitutive (HSP73) and the inducible (HSP72) forms of HSP70.
The MA1-91159 immunogen is: Full length native protein (purified) (Bovine brain).
Store at 4°C for short term. For extended storage, aliquot and store at -20°C or below. Avoid Freeze/Thaw Cycles.
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
87A locus; CG31366-PA; dnaK-type molecular chaperone; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; heat shock 70; heat shock 70 kD protein 1; heat shock 70 kDa protein; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1L; heat shock 70 kDa protein 2; heat shock 70kD protein 1A; heat shock 70kDa protein 1-like; heat shock 70kDa protein 1A; heat shock 70kDa protein 1B; Heat Shock Protein; heat shock protein 1-like; heat shock protein 70; heat shock protein 70-1; heat shock protein-70; heat shock-induced protein; heat shock-induced protein, Hspa70; heat-shock 70-kilodalton protein 1A; heat-shock protein 70; Heat-shock-protein-70Aa; hsp70 87A; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70.1; HSP70.1/2; HSP70.1/HSP70.2; Hsp70Aa-PA; HSP70I; HSP72; HSPA1; protein 70
87A; BOS_21591; CELE_C12C8.1; CG31366; dHsp70; dhsp70Aa; Dm-hsp70; Dmel\CG31366; Dmel_CG31366; DmHSP70AA; HEL-S-103; hs70; Hsp 70; Hsp-70; Hsp-70Aa; HSP70; hsp70 87A; hsp70 Aa; hsp70(87A); HSP70-1; HSP70-1A; Hsp70-2; HSP70.1; Hsp70A; hsp70Aa; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B; Hspa2; HSX70; SBAB-707F1.4