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Immunofluorescent analysis of Heat Shock Protein 70 (Hsp70, green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with (left panel) or without (right panel) a Hsp70 monoclonal antibody (Product # MA3-028), at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with Dylight 554 phalloidin (Product # 21834), and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight at 20X magnification.
|Tested species reactivity||Dog, Human, Mouse, Non-human primate|
|Published species reactivity||Dog, Rat, Non-human primate, Human, Mouse, Guinea pig|
|Host / Isotype||Mouse / IgG3|
|Immunogen||Synthetic peptide corresponding to residues G(615) S G S S G T G E Q K E D Q K E E K Q(633) of mouse mtHSP70.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:50 - 1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1 µg/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-028 detects mitochondrial heat shock protein 70 kDa (mtHSP70) from human, non-human primate, canine and mouse tissues.
MA3-028 has been successfully used in Western blot, immunocytochemical, immunofluorescence, immunohistochemical (paraffin), and immunoprecipitation procedures. By Western blot, this antibody detects a single ~75 kDa band representing mtHSP70 from U2OS cell homogenate. Immunocytochemical staining of mtHSP70 in DAP.3 cells with MA3-028 results in a worm-like staining pattern, consistent with mitochondrial localization.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a 70 kDa protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein which is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa constitutively expressed protein which is involved in uncoating clathrin-coated vesicles; GRP78, or BiP, is a glucose-regulated 78 kDa protein localized to the endoplasmic reticulum; and mitochondrial HSP70 (mtHSP70, GRP75 or mortalin) a 75 kDa protein that is found within the mitochondria.
mtHSP70 is a mitochondrial resident protein that is involved in protein translocation into the mitochondria. Preproteins cross the mitochondrial membranes in an extended conformation. This requires unfolding of preproteins before entering translocation pores in the mitochondrial outer membrane. Preprotein unfolding is thought to be mediated by mtHSP70 and other inner membrane proteins of the mitochondria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Centrosome-intrinsic mechanisms modulate centrosome integrity during fever.
MA3-028 was used in immunocytochemistry to investigate centrosome regulation during fever
|Vertii A,Zimmerman W,Ivshina M,Doxsey S||Molecular biology of the cell (26:3451)||2015|
Diverse intracellular pathogens activate type III interferon expression from peroxisomes.
MA3-028 was used in immunocytochemistry to elucidate type I interferon-independent viral immune responses.
|Odendall C,Dixit E,Stavru F,Bierne H,Franz KM,Durbin AF,Boulant S,Gehrke L,Cossart P,Kagan JC||Nature immunology (15:717)||2014|
An overlapping reading frame in the PRNP gene encodes a novel polypeptide distinct from the prion protein.
MA3-028 was used in immunocytochemistry to study the expression and function of overlapping open reading frames in the prion protein gene PRNP
|Vanderperre B,Staskevicius AB,Tremblay G,McCoy M,O'Neill MA,Cashman NR,Roucou X||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (25:2373)||2011|
CP-31398 restores mutant p53 tumor suppressor function and inhibits UVB-induced skin carcinogenesis in mice.
MA3-028 was used in immunocytochemistry to indicate that reconstituting p53 tumor suppressor functions may inhibit UVB-induced skin carcinogenesis
|Tang X,Zhu Y,Han L,Kim AL,Kopelovich L,Bickers DR,Athar M||The Journal of clinical investigation (117:3753)||2007|
The Mcf1 toxin induces apoptosis via the mitochondrial pathway and apoptosis is attenuated by mutation of the BH3-like domain.
MA3-028 was used in immunocytochemistry to investigate the mechanism for the induction of apoptosis by Mcf1 toxin
|Dowling AJ,Waterfield NR,Hares MC,Le Goff G,Streuli CH,ffrench-Constant RH||Cellular microbiology (9:2470)||2007|
The N-terminal conformation of Bax regulates cell commitment to apoptosis.
MA3-028 was used in immunocytochemistry to study the role of Bax N-terminal conformation in controling the commitment to apoptosis
|Upton JP,Valentijn AJ,Zhang L,Gilmore AP||Cell death and differentiation (14:932)||2007|
Molecular morphology and toxicity of cytoplasmic prion protein aggregates in neuronal and non-neuronal cells.
MA3-028 was used in immunocytochemistry to study the role of cytoplasmic prion protein in prion disease pathogenesis
|Grenier C,Bissonnette C,Volkov L,Roucou X||Journal of neurochemistry (97:1456)||2006|
Caspase inhibitors, but not c-Jun NH2-terminal kinase inhibitor treatment, prevent cisplatin-induced hearing loss.
MA3-028 was used in immunocytochemistry to demonstrate the effect of caspase inhibitors on cisplatin-induced hearing loss.
|Wang J,Ladrech S,Pujol R,Brabet P,Van De Water TR,Puel JL||Cancer research (64:9217)||2004|
Evaluation of the anti-proliferative and anti-oxidative activities of leaf extract from in vivo and in vitro raised Ashwagandha.
MA3-028 was used in immunocytochemistry to investigate the effect of leaf extract from Ashwagandha on cell proliferation and oxidative status
|Kaur K,Rani G,Widodo N,Nagpal A,Taira K,Kaul SC,Wadhwa R||Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (42:2015)||2004|
Tid1, a cochaperone of the heat shock 70 protein and the mammalian counterpart of the Drosophila tumor suppressor l(2)tid, is critical for early embryonic development and cell survival.
MA3-028 was used in immunocytochemistry to study the function of Tid1 during early mammalian development.
|Lo JF,Hayashi M,Woo-Kim S,Tian B,Huang JF,Fearns C,Takayama S,Zapata JM,Yang Y,Lee JD||Molecular and cellular biology (24:2226)||2004|
The mitochondrial barriers segregate agonist-induced calcium-dependent functions in human airway epithelia.
MA3-028 was used in immunocytochemistry to investigate the distribution of mitochondria in polarized human airway epithelia
|Ribeiro CM,Paradiso AM,Livraghi A,Boucher RC||The Journal of general physiology (122:377)||2003|
Spatial and temporal changes in Bax subcellular localization during anoikis.
MA3-028 was used in immunocytochemistry to find the relationship between Bax activation complex formation and apoptosis
|Valentijn AJ,Metcalfe AD,Kott J,Streuli CH,Gilmore AP||The Journal of cell biology (162:599)||2003|
|Non-human primate||Not Cited||
Isolation and expression of a novel mitochondrial septin that interacts with CRMP/CRAM in the developing neurones.
MA3-028 was used in immunocytochemistry to identify a CRMP/CRAM interacting protein septin in mitochondria
|Takahashi S,Inatome R,Yamamura H,Yanagi S||Genes to cells : devoted to molecular & cellular mechanisms (8:81)||2003|
Alix (ALG-2-interacting protein X), a protein involved in apoptosis, binds to endophilins and induces cytoplasmic vacuolization.
MA3-028 was used in immunocytochemistry to investigate the role of Alix during the cytoplasmic vacuolization in apoptosis.
|Chatellard-Causse C,Blot B,Cristina N,Torch S,Missotten M,Sadoul R||The Journal of biological chemistry (277:29108)||2002|
Glutathione levels and BAX activation during apoptosis due to oxidative stress in cells expressing wild-type and mutant cystic fibrosis transmembrane conductance regulator.
MA3-028 was used in immunocytochemistry to investigate what regulates the glutathione levels and BAX activation during apoptosis.
|Jungas T,Motta I,Duffieux F,Fanen P,Stoven V,Ojcius DM||The Journal of biological chemistry (277:27912)||2002|
The tripartite motif family identifies cell compartments.
MA3-028 was used in immunocytochemistry to demonstrate that the tripartite motif proteins identify specific cell compartments
|Reymond A,Meroni G,Fantozzi A,Merla G,Cairo S,Luzi L,Riganelli D,Zanaria E,Messali S,Cainarca S,Guffanti A,Minucci S,Pelicci PG,Ballabio A||The EMBO journal (20:2140)||2001|
Calcium regulates the association between mitochondria and a smooth subdomain of the endoplasmic reticulum.
MA3-028 was used in immunocytochemistry to demonstrate the role of calcium in mitochondria and endoplasmic reticulum association.
|Wang HJ,Guay G,Pogan L,Sauvé R,Nabi IR||The Journal of cell biology (150:1489)||2000|
The BARD1 BRCT domain contributes to p53 binding, cytoplasmic and mitochondrial localization, and apoptotic function.
MA3-028 was used in western blot to describe the localization of BARD1 and study its interaction with p53
|Tembe V,Martino-Echarri E,Marzec KA,Mok MT,Brodie KM,Mills K,Lei Y,DeFazio A,Rizos H,Kettle E,Boadle R,Henderson BR||Cellular signalling (27:1763)||2015|
Matrine suppresses proliferation and induces apoptosis in human cholangiocarcinoma cells through suppression of JAK2/STAT3 signaling.
MA3-028 was used in western blot to assess the effect of matrine on STAT3 signaling in cholangiocarcinoma cells
|Yang N,Han F,Cui H,Huang J,Wang T,Zhou Y,Zhou J||Pharmacological reports : PR (67:388)||2015|
Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling.
MA3-028 was used in western blot to study phosphorylation events at sites of adhesion
|Robertson J,Jacquemet G,Byron A,Jones MC,Warwood S,Selley JN,Knight D,Humphries JD,Humphries MJ||Nature communications (6:null)||2015|
Promotion of p53 expression and reactive oxidative stress production is involved in zerumbone-induced cisplatin sensitization of non-small cell lung cancer cells.
MA3-028 was used in western blot to determine the effect of zerumbone on p53 signaling and assess if zerumbone has anti-cancer effects on non-small cell lung cancer
|Hu Z,Zeng Q,Zhang B,Liu H,Wang W||Biochimie (107 Pt B:257)||2014|
Role of p53 in cAMP/PKA pathway mediated apoptosis.
MA3-028 was used in western blot to study the role of p53 in apoptosis mediated by the cAMP/PKA signaling pathway
|Rahimi A,Lee YY,Abdella H,Doerflinger M,Gangoda L,Srivastava R,Xiao K,Ekert PG,Puthalakath H||Apoptosis : an international journal on programmed cell death (18:1492)||2013|
Neurobeachin regulates neurotransmitter receptor trafficking to synapses.
MA3-028 was used in western blot to study the mechanism by which neurobeachin regulates synaptic transmission
|Nair R,Lauks J,Jung S,Cooke NE,de Wit H,Brose N,Kilimann MW,Verhage M,Rhee J||The Journal of cell biology (200:61)||2013|
HIPK2 controls cytokinesis and prevents tetraploidization by phosphorylating histone H2B at the midbody.
MA3-028 was used in western blot to study the role of HIPK2 in the control of cytokinesis and preventing tetraploidization
|Rinaldo C,Moncada A,Gradi A,Ciuffini L,D'Eliseo D,Siepi F,Prodosmo A,Giorgi A,Pierantoni GM,Trapasso F,Guarguaglini G,Bartolazzi A,Cundari E,Schininà ME,Fusco A,Soddu S||Molecular cell (47:87)||2012|
Fluoxetine affects hippocampal plasticity, apoptosis and depressive-like behavior of chronically isolated rats.
MA3-028 was used in western blot to investigate the effect of fluoxetine on hippocampal plasticity, apoptosis and depressive-like behavior in rats
|Djordjevic A,Djordjevic J,Elaković I,Adzic M,Matić G,Radojcic MB||Progress in neuro-psychopharmacology & biological psychiatry (36:92)||2012|
Dual function of protein kinase C (PKC) in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced manganese superoxide dismutase (MnSOD) expression: activation of CREB and FOXO3a by PKC-alpha phosphorylation and by PKC-mediated inactivation of Akt, respectively.
MA3-028 was used in western blot to investigate the effect of PKC on MnSOD expression in human cells
|Chung YW,Kim HK,Kim IY,Yim MB,Chock PB||The Journal of biological chemistry (286:29681)||2011|
Low-dose valproic acid enhances radiosensitivity of prostate cancer through acetylated p53-dependent modulation of mitochondrial membrane potential and apoptosis.
MA3-028 was used in western blot to investigate the mechanism for cancer radiosensitization induced by histone deacetylase inhibitors
|Chen X,Wong JY,Wong P,Radany EH||Molecular cancer research : MCR (9:448)||2011|
A NH2 tau fragment targets neuronal mitochondria at AD synapses: possible implications for neurodegeneration.
MA3-028 was used in western blot to investigate the role of a NH2 tau fragment in neuronal mitochondria
|Amadoro G,Corsetti V,Stringaro A,Colone M,D'Aguanno S,Meli G,Ciotti M,Sancesario G,Cattaneo A,Bussani R,Mercanti D,Calissano P||Journal of Alzheimer's disease : JAD (21:445)||2010|
Different expression of alpha and beta mitochondrial estrogen receptors in the aging rat brain: interaction with respiratory complex V.
MA3-028 was used in western blot to investigate the distribution of mitochondrial estrogen receptor alpha and beta in the aging rat brain
|Alvarez-Delgado C,Mendoza-Rodríguez CA,Picazo O,Cerbón M||Experimental gerontology (45:580)||2010|
Chronic social isolation compromises the activity of both glutathione peroxidase and catalase in hippocampus of male wistar rats.
MA3-028 was used in western blot to investigate the effect of chronic social isolation on the activity of glutathione peroxidase and catalase in the hippocampus of male wistar rats
|Djordjevic J,Djordjevic A,Adzic M,Radojcic MB||Cellular and molecular neurobiology (30:693)||2010|
Amino terminal hydrophobic import signals target the p14(ARF) tumor suppressor to the mitochondria.
MA3-028 was used in western blot to investigate the mitochondrial targeting of p14ARF tumor suppressor
|Irvine M,Philipsz S,Frausto M,Mijatov B,Gallagher SJ,Fung C,Becker TM,Kefford RF,Rizos H||Cell cycle (Georgetown, Tex.) (9:829)||2010|
Chronic social isolation is related to both upregulation of plasticity genes and initiation of proapoptotic signaling in Wistar rat hippocampus.
MA3-028 was used in western blot to study the effect of chronic social isolation on neuronal plasticity and apoptosis
|Djordjevic A,Adzic M,Djordjevic J,Radojcic MB||Journal of neural transmission (Vienna, Austria : 1996) (116:1579)||2009|
The role of phosphorylated glucocorticoid receptor in mitochondrial functions and apoptotic signalling in brain tissue of stressed Wistar rats.
MA3-028 was used in western blot to study the mitochondrial dysfunction in neuroendocrine stresses and its mechanism
|Adzic M,Djordjevic A,Demonacos C,Krstic-Demonacos M,Radojcic MB||The international journal of biochemistry & cell biology (41:2181)||2009|
Loss of anti-Bax function in Gerstmann-Sträussler-Scheinker syndrome-associated prion protein mutants.
MA3-028 was used in western blot to study the role of the anti-Bax function of PrP in prion disease pathology
|Jodoin J,Misiewicz M,Makhijani P,Giannopoulos PN,Hammond J,Goodyer CG,LeBlanc AC||PloS one (4:null)||2009|
MDM4 (MDMX) localizes at the mitochondria and facilitates the p53-mediated intrinsic-apoptotic pathway.
MA3-028 was used in western blot to study the role of mitochondrial MDM4 in regulating the p53-mediated intrinsic-apoptotic pathway
|Mancini F,Di Conza G,Pellegrino M,Rinaldo C,Prodosmo A,Giglio S,D'Agnano I,Florenzano F,Felicioni L,Buttitta F,Marchetti A,Sacchi A,Pontecorvi A,Soddu S,Moretti F||The EMBO journal (28:1926)||2009|
Mitochondrial Lon protease is a human stress protein.
MA3-028 was used in western blot to study the induction the mitochondrial Lon protease by multiple cellular stressors and its role as a general stress-protector
|Ngo JK,Davies KJ||Free radical biology & medicine (46:1042)||2009|
Helix 3 is necessary and sufficient for prion protein's anti-Bax function.
MA3-028 was used in western blot to study the role of helix 3 in the anti-Bax function of prion protein
|Laroche-Pierre S,Jodoin J,LeBlanc AC||Journal of neurochemistry (108:1019)||2009|
Targets of caspase-6 activity in human neurons and Alzheimer disease.
MA3-028 was used in western blot to identify those components regulated by caspase-6 in human neurons
|Klaiman G,Petzke TL,Hammond J,Leblanc AC||Molecular & cellular proteomics : MCP (7:1541)||2008|
Proteomics-based identification of autoantibody against heat shock protein 70 as a diagnostic marker in esophageal squamous cell carcinoma.
MA3-028 was used in western blot to investigate an autoantibody against Hsp70 in esophageal squamous cell carcinoma patients
|Fujita Y,Nakanishi T,Miyamoto Y,Hiramatsu M,Mabuchi H,Miyamoto A,Shimizu A,Takubo T,Tanigawa N||Cancer letters (263:280)||2008|
Chronic exposure to sub-lethal beta-amyloid (Abeta) inhibits the import of nuclear-encoded proteins to mitochondria in differentiated PC12 cells.
MA3-028 was used in western blot to investigate the influence of Abeta on protein transport from nucleus to mitochondira
|Sirk D,Zhu Z,Wadia JS,Shulyakova N,Phan N,Fong J,Mills LR||Journal of neurochemistry (103:1989)||2007|
Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase.
MA3-028 was used in western blot to study the regulation of insulin secretion by SIRT4
|Ahuja N,Schwer B,Carobbio S,Waltregny D,North BJ,Castronovo V,Maechler P,Verdin E||The Journal of biological chemistry (282:33583)||2007|
Defective retrotranslocation causes loss of anti-Bax function in human familial prion protein mutants.
MA3-028 was used in western blot to study the mechanism of the anti-Bax function of familial PrP mutants
|Jodoin J,Laroche-Pierre S,Goodyer CG,LeBlanc AC||The Journal of neuroscience : the official journal of the Society for Neuroscience (27:5081)||2007|
Dissociating the dual roles of apoptosis-inducing factor in maintaining mitochondrial structure and apoptosis.
MA3-028 was used in western blot to dissociate the dual functions of apoptosis-inducing factor (AIF) in controlling mitochondrial structure and apoptosis
|Cheung EC,Joza N,Steenaart NA,McClellan KA,Neuspiel M,McNamara S,MacLaurin JG,Rippstein P,Park DS,Shore GC,McBride HM,Penninger JM,Slack RS||The EMBO journal (25:4061)||2006|
WT p53, but not tumor-derived mutants, bind to Bcl2 via the DNA binding domain and induce mitochondrial permeabilization.
MA3-028 was used in western blot to study the interaction between wild-type p53 and Bcl2 and its effect on mitochondrial permeabilization.
|Tomita Y,Marchenko N,Erster S,Nemajerova A,Dehner A,Klein C,Pan H,Kessler H,Pancoska P,Moll UM||The Journal of biological chemistry (281:8600)||2006|
Hepatitis C virus core protein inhibits mitochondrial electron transport and increases reactive oxygen species (ROS) production.
MA3-028 was used in western blot to demonstrate the effect of hepatitis C virus core protein on mitochondrial electron transport and reactive oxygen species production.
|Korenaga M,Wang T,Li Y,Showalter LA,Chan T,Sun J,Weinman SA||The Journal of biological chemistry (280:37481)||2005|
Viral and cellular oncogenes induce rapid mitochondrial translocation of p53 in primary epithelial and endothelial cells early in apoptosis.
MA3-028 was used in western blot to study the role of viral and cellular oncogenes in the mitochondrial translocation of p53 in apoptosis
|Nemajerova A,Wolff S,Petrenko O,Moll UM||FEBS letters (579:6079)||2005|
Tumor cell pseudopodial protrusions. Localized signaling domains coordinating cytoskeleton remodeling, cell adhesion, glycolysis, RNA translocation, and protein translation.
MA3-028 was used in western blot to demonstrate the role of Rho/ROCK activation in RNA translocation to pseudopodial protrusions in epithelial tumor cells.
|Jia Z,Barbier L,Stuart H,Amraei M,Pelech S,Dennis JW,Metalnikov P,O'Donnell P,Nabi IR||The Journal of biological chemistry (280:30564)||2005|
Cellular prion protein inhibits proapoptotic Bax conformational change in human neurons and in breast carcinoma MCF-7 cells.
MA3-028 was used in western blot to study the inhibition of the proapoptotic Bax conformational change by cellular prion protein in human neurons and breast carcinoma cells
|Roucou X,Giannopoulos PN,Zhang Y,Jodoin J,Goodyer CG,LeBlanc A||Cell death and differentiation (12:783)||2005|
53BP2 induces apoptosis through the mitochondrial death pathway.
MA3-028 was used in western blot to investigate the role of 53BP2 in the mitochondrial death pathway
|Kobayashi S,Kajino S,Takahashi N,Kanazawa S,Imai K,Hibi Y,Ohara H,Itoh M,Okamoto T||Genes to cells : devoted to molecular & cellular mechanisms (10:253)||2005|
Human mitochondrial complex I assembles through the combination of evolutionary conserved modules: a framework to interpret complex I deficiencies.
MA3-028 was used in western blot to study the assembly mechanism of human mitochondrial complex I.
|Ugalde C,Vogel R,Huijbens R,Van Den Heuvel B,Smeitink J,Nijtmans L||Human molecular genetics (13:2461)||2004|
Translocation of full-length Bid to mitochondria during anoikis.
MA3-028 was used in western blot to investigate the role of Bid in anoikis.
|Valentijn AJ,Gilmore AP||The Journal of biological chemistry (279:32848)||2004|
Stress induces mitochondria-mediated apoptosis independent of SAPK/JNK activation in embryonic stem cells.
MA3-028 was used in western blot to investigate the mechanism of the mitochondria-mediated apoptosis in embryonic stem cells.
|Nishitai G,Shimizu N,Negishi T,Kishimoto H,Nakagawa K,Kitagawa D,Watanabe T,Momose H,Ohata S,Tanemura S,Asaka S,Kubota J,Saito R,Yoshida H,Mak TW,Wada T,Penninger JM,Azuma N,Nishina H,Katada T||The Journal of biological chemistry (279:1621)||2004|
Mitochondrial biogenesis and remodeling during adipogenesis and in response to the insulin sensitizer rosiglitazone.
MA3-028 was used in western blot to study the mitochondrial alterations during adipocyte differentiation
|Wilson-Fritch L,Burkart A,Bell G,Mendelson K,Leszyk J,Nicoloro S,Czech M,Corvera S||Molecular and cellular biology (23:1085)||2003|
Dual localization of human DNA topoisomerase IIIalpha to mitochondria and nucleus.
MA3-028 was used in western blot to study mitochondrial and nucleic localization of human DNA topoisomerase IIIalpha.
|Wang Y,Lyu YL,Wang JC||Proceedings of the National Academy of Sciences of the United States of America (99:12114)||2002|
The human silent information regulator (Sir)2 homologue hSIRT3 is a mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase.
MA3-028 was used in western blot to characterize a new mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase .
|Schwer B,North BJ,Frye RA,Ott M,Verdin E||The Journal of cell biology (158:647)||2002|
Late activation of apoptotic pathways plays a negligible role in mediating the cytotoxic effects of discodermolide and epothilone B in non-small cell lung cancer cells.
MA3-028 was used in western blot to investigate the role of late activation of apoptotic pathway in mediating the cytotoxic effects of discodermolide and epothilone B in non-small cell lung cancer cells.
|Bröker LE,Huisman C,Ferreira CG,Rodriguez JA,Kruyt FA,Giaccone G||Cancer research (62:4081)||2002|
The course of etoposide-induced apoptosis from damage to DNA and p53 activation to mitochondrial release of cytochrome c.
MA3-028 was used in western blot to investigate the sequence of biochemical events that mediates the apoptosis induced by etoposide.
|Karpinich NO,Tafani M,Rothman RJ,Russo MA,Farber JL||The Journal of biological chemistry (277:16547)||2002|
68 kDa heat shock protein; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; heat shock 70 kDa protein 3; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock protein, 70 kDa 3; heat shock-induced protein; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70.1/HSP70.2; HSP70I; HSP72; HSPA1; inducible heat shock protein 70
HEL-S-103; hsp68; HSP70-1; HSP70-1A; Hsp70-3; HSP70.1; Hsp70.3; Hsp70A1; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B; HSX70