A549 cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ON-TARGETplus HSPA1A siRNA (Lane 3). Western blot data for GAPDH is included as a control for equal protein loading.
|Tested species reactivity||Bovine, Human, Non-human primate|
|Published species reactivity||Dog, Hamster, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||HSP70 protein from HeLa cells.|
|Storage buffer||PBS, pH 7.2, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 ug/ml|
|Immunoprecipitation (IP)||2 µg/mg|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-90504 detects Hsp70 from human, non-human primate, bovine samples.
MA1-90504 has been successfully used in flow cytometry, immunofluorescence, immunohistochemistry (paraffin tissue), immunoprecipitation, Western blot applications.
The MA1-90504 immunogen is: HSP70 protein from HeLa cells.
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Oncogenic acidic nuclear phosphoproteins ANP32C/D are novel clients of heat shock protein 90.
MA1-90504 was used in western blot to investigate ANP32C-Hsp90 interactions.
|Yuzefovych Y,Blasczyk R,Huyton T||Biochimica et biophysica acta (1853:2338)||2015|
|Not Applicable||Not Cited||
New cell culture model for aromatase inhibitor-resistant breast cancer shows sensitivity to fulvestrant treatment and cross-resistance between letrozole and exemestane.
MA1-90504 was used in western blot to use a cell culture model for aromatase inhibitor-resistant breast cancer to show how fulvestrant treatment and cross-resistance between exemestane and letrozole shows sensitivity
|Hole S,Pedersen AM,Hansen SK,Lundqvist J,Yde CW,Lykkesfeldt AE||International journal of oncology (46:1481)||2015|
T47D breast cancer cells switch from ER/HER to HER/c-Src signaling upon acquiring resistance to the antiestrogen fulvestrant.
MA1-90504 was used in western blot to study the switch from ER/HER to HER/c-Src signaling as T47D breast cancer cells acquire fulvestrant resistance
|Kirkegaard T,Hansen SK,Larsen SL,Reiter BE,Sørensen BS,Lykkesfeldt AE||Cancer letters (344:90)||2014|
The apoptosis repressor with a CARD domain (ARC) gene is a direct hypoxia-inducible factor 1 target gene and promotes survival and proliferation of VHL-deficient renal cancer cells.
MA1-90504 was used in western blot to study the role of HIF-1alpha-induced ARC expression in VHL-deficient renal cancer cell survival and proliferation
|Razorenova OV,Castellini L,Colavitti R,Edgington LE,Nicolau M,Huang X,Bedogni B,Mills EM,Bogyo M,Giaccia AJ||Molecular and cellular biology (34:739)||2014|
Heat-shock factor 1 both positively and negatively affects cellular clonogenic growth depending on p53 status.
MA1-90504 was used in western blot to study the effects of p53 status on the modulation of clonogenic cellular growth by HSF-1
|Nguyen CH,Lang BJ,Chai RC,Vieusseux JL,Kouspou MM,Price JT||The Biochemical journal (452:321)||2013|
Estrogen receptor ¿ is the major driving factor for growth in tamoxifen-resistant breast cancer and supported by HER/ERK signaling.
MA1-90504 was used in western blot to study the key role of ER-alpha and the supporting roles of HER and ERK in the growth of breast cancer cells resistant to tamoxifen
|Thrane S,Lykkesfeldt AE,Larsen MS,Sorensen BS,Yde CW||Breast cancer research and treatment (139:71)||2013|
Mechanism-based screen for G1/S checkpoint activators identifies a selective activator of EIF2AK3/PERK signalling.
MA1-90504 was used in western blot to identify and characterize small molecule inhibitors of G1/S checkpoint activation
|Stockwell SR,Platt G,Barrie SE,Zoumpoulidou G,Te Poele RH,Aherne GW,Wilson SC,Sheldrake P,McDonald E,Venet M,Soudy C,Elustondo F,Rigoreau L,Blagg J,Workman P,Garrett MD,Mittnacht S||PloS one (7:null)||2012|
Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
MA1-90504 was used in western blot to investigate the dynamics of lysine ubiquitination
|Xu G,Paige JS,Jaffrey SR||Nature biotechnology (28:868)||2010|
Expression of the Longin domain of TI-VAMP impairs lysosomal secretion and epithelial cell migration.
MA1-90504 was used in western blot to investigate the effect of TI-VAMP Longin domain on lysosomal secretion and epithelial cell migration
|Proux-Gillardeaux V,Raposo G,Irinopoulou T,Galli T||Biology of the cell (99:261)||2007|
Insulin-like growth factor binding protein 2 is a marker for antiestrogen resistant human breast cancer cell lines but is not a major growth regulator.
MA1-90504 was used in western blot to study the role of IGFBP-2 as a marker of anti-estrogen-resistant breast cancer cell lines
|Juncker-Jensen A,Lykkesfeldt AE,Worm J,Ralfkiaer U,Espelund U,Jepsen JS||Growth hormone and IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society (16:224)||2006|
Purification of recombinant and endogenous HSP70s.
MA1-90504 was used in western blot to describe a method for the purification of endogenous and recombinant HSP70 proteins
|Ménoret A||Methods (San Diego, Calif.) (32:7)||2004|
The Fas-induced apoptosis analyzed by high throughput proteome analysis.
MA1-90504 was used in western blot to perform a proteomic analysis of Jurkat T-cell cytosolic proteins during Fas-induced cell death
|Gerner C,Frohwein U,Gotzmann J,Bayer E,Gelbmann D,Bursch W,Schulte-Hermann R||The Journal of biological chemistry (275:39018)||2000|
Correlation between depression, anxiety, and polymorphonuclear cells' resilience in ulcerative colitis: the mediating role of heat shock protein 70.
MA1-90504 was used in immunohistochemistry to study the correlation between hsp70 levels in polymorphonuclear cells and depression/anxiety in patients with ulcerative colitis
|Vlachos II,Barbatis C,Tsopanomichalou M,Abou-Assabeh L,Goumas K,Ginieri-Coccossis M,Economou M,Papadimitriou GN,Patsouris E,Nicolopoulou-Stamati P||BMC gastroenterology (14:null)||2014|
Heat shock protein 70 (HSP70) expression is associated with poor prognosis in intestinal type gastric cancer.
MA1-90504 was used in immunohistochemistry to study the prognostic value of the immunohistochemical expression of Hsp70 in intestinal-type gastric cancer
|Lee HW,Lee EH,Kim SH,Roh MS,Jung SB,Choi YC||Virchows Archiv : an international journal of pathology (463:489)||2013|
Immunohistochemical expression of heat shock protein 70 in vitiligo.
MA1-90504 was used in immunohistochemistry to study the immunohistochemical expression of Hsp70 in normal and vitiliginous skin and its role in vitiligo pathogenesis
|Abdou AG,Maraee AH,Reyad W||Annals of diagnostic pathology (17:245)||2013|
Comparison of the expression of 5 heat shock proteins in benign and malignant salivary gland tumor tissues.
MA1-90504 was used in immunohistochemistry to study benign and malignant salivary gland tumors for the expression of a range of heat shock proteins
|Wang G,Gu X,Chen L,Wang Y,Cao B,E Q||Oncology letters (5:1363)||2013|
Expressions of HSP70 and HSP27 in hepatocellular carcinoma.
MA1-90504 was used in immunohistochemistry to study the expressions of heat shock proteins in hepatocellular carcinoma and their role in carcinogenesis
|Joo M,Chi JG,Lee H||Journal of Korean medical science (20:829)||2005|
Heat shock proteins 60 and 70 expression of cutaneous lichen planus: comparison with normal skin and psoriasis vulgaris.
MA1-90504 was used in immunohistochemistry to investigate the different expression of heat shock protein -60,-70 in normal skin and psoriasis vulgaris
|Bayramgürler D,Ozkara SK,Apaydin R,Erçin C,Bilen N||Journal of cutaneous pathology (31:586)||2004|
The cochaperone Bag-1L enhances androgen receptor action via interaction with the NH2-terminal region of the receptor.
MA1-90504 was used in immunohistochemistry to study the mechanism by which the co-chaperone Bag-1L enhances androgen receptor action
|Shatkina L,Mink S,Rogatsch H,Klocker H,Langer G,Nestl A,Cato AC||Molecular and cellular biology (23:7189)||2003|
Immunohistochemical study of heat shock proteins 27, 60 and 70 in the normal human adrenal and in adrenal tumors with suppressed ACTH production.
MA1-90504 was used in immunohistochemistry to investigate the expression of heat shock proteins in normal and tumoral adrenal glands
|Pignatelli D,Ferreira J,Soares P,Costa MJ,Magalhães MC||Microscopy research and technique (61:315)||2003|
Expression of heat-shock proteins hsp27, hsp70 and hsp90 in malignant epithelial tumour of the ovaries.
MA1-90504 was used in immunohistochemistry to study the expression of heat-shock proteins in malignant epithelial ovarian tumors
|Elpek GO,Karaveli S,Sim¿ek T,Keles N,Aksoy NH||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (111:523)||2003|
Intracellular clusterin causes juxtanuclear aggregate formation and mitochondrial alteration.
MA1-90504 was used in immunocytochemistry to study the role of intracellular clusterin in juxtanuclear aggregate formation and mitochondrial alteration
|Debure L,Vayssiere JL,Rincheval V,Loison F,Le Drean Y,Michel D||Journal of cell science (116:3109)||2003|