|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 308 and 641 of Human HSP70 1A|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-28003 targets HSP70 1A in immunocytochemistry, immunofluorescence, Western blot, immunohistochemistry (paraffin), and immunoprecipitation applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-28003 immunogen is recombinant protein fragment corresponding to a region within amino acids 308 and 641 of Human HSP70 1A.
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Differential Proteomic Analysis of Syncytiotrophoblast Extracellular Vesicles from Early-Onset Severe Preeclampsia, using 8-Plex iTRAQ Labeling Coupled with 2D Nano LC-MS/MS.
PA5-28003 was used in western blot to identify proteins in syncytiotrophoblast extracellular vesicles and determine their role in preeclampsia.
|Li H,Han L,Yang Z,Huang W,Zhang X,Gu Y,Li Y,Liu X,Zhou L,Hu J,Yu M,Yang J,Li Y,Zheng Y,Guo J,Han J,Li L||Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (36:1116)||2015|