|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:25|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to aa 574-591 of human HSP70|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.
The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75 and GRP78. These proteins serve a variety of roles: they act as molecular chaperones facilitating the assembly of multi-protein complexes, they participate in the translocation of polypeptides across cell membranes and to the nucleus and they aid in the proper folding of nascent polypeptide chain. HSP70 expression is strongly induced in response to heat stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; Heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; heat shock-induced protein, Hspa70; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-2; HSP70.1/HSP70.2; HSP70I; HSP72; HSPA1
Gene Aliases: HEL-S-103; HSP70-1; HSP70-1A; HSP70.1; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B; HSX70
UniProt ID: (Human) P0DMV8
Entrez Gene ID: (Human) 3303
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