|Tested species reactivity||Bovine, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Heat Shock Protein 90|
|Storage buffer||PBS, pH 7.2, with 1% BSA, 50% glycerol|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||10-20 µg/ml|
|Immunoprecipitation (IP)||4 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-26361 detects Heat Shock Protein 90 from bovine, human, mouse, porcine, rabbit, rat, ovine, and monkey samples.
MA1-26361 has been successfully used in immunoprecipitation, Immunohistochemistry (paraffin), and Western blot procedures. Detects molecular weight band of approximately 90 kDa.
The MA1-26361 immunogen is Heat Shock Protein 90.
Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, aryl hydrocarbon (Ah) receptor, and some kinases.
Studies have shown that murine HSP90 exists as two forms, HSP84 and HSP86, coded by related but separate genes, with 86% amino acid sequence conservation. These forms are analogous to the two forms of human HSP90, HSP89 alpha and HSP89 beta. In an unstressed mouse fibroblast, the basal level of HSP84 is found to be double that of HSP86. However, after heat shock, HSP86 shows a greater increase. Studies also suggest that upon cellular differentiation, the level of HSP86, but not HSP84, decreases. HSP84 and HSP86, which may be subject to estrogenic regulation, have been found as components of the non-DNA binding form of mouse glucocorticoid receptor, but dissociated from the transformed DNA-binding form.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.