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|Tested species reactivity||Human, Mouse, Rabbit|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Purified human Hsp90 beta.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1-5 µg/ml|
|Immunoprecipitation (IP)||10 µg per 100 µl sample|
|Western Blot (WB)||1-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-10892 detects Heat Shock Protein 90 alpha and beta in human, mouse and rabbit samples.
MA1-10892 has been successfully used in Western blot, immunohistochemistry and immunoprecipitation procedures. By Western blot, MA1-10892 is specific for the ~90 kDa Heat Shock Protein 90 protein.
MA1-10892 immunogen is purified human Hsp90 beta.
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 ., a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A C-terminal HSP90 inhibitor restores glucocorticoid sensitivity and relieves a mouse allograft model of Cushing disease.
MA1-10892 was used in western blot to investigate how HSP90 affects the glucocorticoid receptor in corticotroph cells.
|Riebold M,Kozany C,Freiburger L,Sattler M,Buchfelder M,Hausch F,Stalla GK,Paez-Pereda M||Nature medicine (21:276)||2015|
GCUNC45 is the first Hsp90 co-chaperone to show alpha/beta isoform specificity.
MA1-10892 was used in immunocytochemistry and western blot to study the isoform-specificity of Hsp90 co-chaperone GCUNC45
|Chadli A,Felts SJ,Toft DO||The Journal of biological chemistry (283:9509)||2008|
The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties.
MA1-10892 was used in immunoprecipitation to investigate the functional properties HSP90-related protein TRAP1 in mitochondria
|Felts SJ,Owen BA,Nguyen P,Trepel J,Donner DB,Toft DO||The Journal of biological chemistry (275:3305)||2000|
90-kda heat shock protein beta HSP90 beta; 94 kDa glucose-regulated protein; alpha; alpha-like 4; beta; D6S182; endoplasmic reticulum resident protein 99; endoplasmin; epididymis luminal secretory protein 52; epididymis secretory sperm binding protein Li 65p; heat shock 84 kDa; heat shock 86 kDa; heat shock 90kD protein; heat shock 90kD protein 1; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein 1, beta; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1; heat shock 90kDa protein 1, alpha; heat shock protein 1, alpha; heat shock protein 90 kDa; heat shock protein 90 kDa beta member 1; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha (cytosolic), class B member 1; heat shock protein 90kDa alpha family class A member 1; heat shock protein 90kDa alpha family class B member 1; heat shock protein beta; heat shock protein HSP 90-alpha; heat shock protein HSP 90-beta; heat shock protein, 1; heat shock protein, 86 kDa 1; heat shock protein, 89 kDa; HSP 84; HSP 86; HSP84; HSP86; Hsp89; HSP89A; Hsp90; HSP90-BETA; HSP90A; HSP90B; HSP90N; HSPC1; HSPC2; HSPCA; HSPCAL1; HSPCAL4; HSPCB; HSPN; LAP2; lipopolysaccharide-associated protein 2; LPS-associated protein 2; polymorphic tumor rejection antigen 1; renal carcinoma antigen NY-REN-38; transforming growth factor alpha regulated gene 2; TSTA; tumor rejection antigen (gp96) 1; tumor rejection antigen gp96; tumor-specific transplantation 86 kDa antigen
86kDa; 89kDa; AL024080; AL024147; D6S182; EL52; endoplasmin; ERp99; gp96; GRP94; HEL-S-65p; hsp4; HSP84; HSP86; Hsp86-1; Hsp89; HSP89A; Hsp90; HSP90A; HSP90AA1; HSP90AB1; HSP90B; Hsp90b1; HSP90N; HSPC1; HSPC2; HSPCA; HSPCAL1; HSPCAL4; HSPCB; HSPN; LAP-2; LAP2; TA-3; Targ2; Tra-1; Tra1