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HSP90 Antibody (MA5-45106) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with Hsp90 complex monoclonal antibody (Product # MA5-45106) at 1:100 for 12 hours at 4°C, followed by R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 complex antibody. (C) Composite.
Product Details
MA5-45106
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
8D3
Immunogen
Ah receptor (Aryl hydrocarbon receptor)
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Goat anti-mouse IgM was used to bind 25 µl of protein G-Sepharose. MA5-45106 IgM from 0.5 ml of high speed supernatant medium was loaded onto the IgG resin and incubated with 100 µl of rabbit reticulocyte lysate for 30 min. at 30C. After washing (4X1 ml), bound proteins were resolved on SDS PAGE, including HSP90, HSP70 and Hop. |Detects 90kDa. Co-immunoprecipitates HSP90 complexes, including HSP70, Hop, Ah receptors, glucocorticoid receptors, heme-regulated eukaryotic initiation factor 2 alpha (eIF-2 alpha ) kinase (HRI).
Target Information
HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 and mitochondria (Chen et al., 2005 ).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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