Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
|Tested species reactivity||Fish , Mouse , Human , Rat , Chicken , Primate|
|Published species reactivity||Fruit fly , Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||Purified mouse HSP90.|
|Storage buffer||ascites with Ammonium sulfate|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:50 - 1:200|
|Immunoprecipitation (IP)||1:500 - 1:1000|
|Western Blot (WB)||1:200 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA3-011 detects heat shock protein 90 kDa (HSP90) from human, mouse, rat, rainbow trout, and chicken tissues.
MA3-011 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 90 kDa protein representing HSP90 from Hepa 1 cell extract. It has been reported that up to 90% of cytosolic HSP90 can be immunoprecipitated with this product under low ionic strength conditions.
For Western blots using MA3-011, nitrocellulose is recommended. PVDF membranes typically do not yield acceptable results with this antibody.
Before use, centrifuge suspension (4000 g for 15 minutes) and discard supernatant. Resuspend pellet in 200µl of PBS, pH 7.2. If desired, 0.05% sodium azide may be added to resuspended solution. Removal of residual ammonium sulfate is not usually necessary for immunoprecipitation and Western blot procedures, however, any other uses may require its removal by dialysis or equivalent method. Stable for 6 months after PBS resuspension.
The MA3-011 antigen is purified HSP90 from Hepa 1 cytosol.
Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hsp90 regulates the phosphorylation and activity of serum- and glucocorticoid-regulated kinase-1.
MA3-011 was used in immunoprecipitation to investigate the role of Hsp90 in the PI3K/SGK-1 pathway.
|Belova L,Brickley DR,Ky B,Sharma SK,Conzen SD||The Journal of biological chemistry (283:18821)||2008|
Cytoplasmic localization of pregnane X receptor and ligand-dependent nuclear translocation in mouse liver.
MA3-011 was used in immunoprecipitation to investigate the effect of cytoplasmic constitutive active/androstane receptor retention protein (CCRP) on pregnane X receptor localization.
|Squires EJ,Sueyoshi T,Negishi M||The Journal of biological chemistry (279:49307)||2004|
The hsp90 chaperone complex regulates intracellular localization of the dioxin receptor.
MA3-011 was used in immunoprecipitation to investigate the role of the hsp90 molecular chaperone complex in the intracellular localization of the dioxin receptor.
|Kazlauskas A,Sundström S,Poellinger L,Pongratz I||Molecular and cellular biology (21:2594)||2001|
|Fruit fly||Not Cited||
The basic helix-loop-helix/PAS factor Sim is associated with hsp90. Implications for regulation by interaction with partner factors.
MA3-011 was used in immunoprecipitation to study the interaction between Sim and hsp90
|McGuire J,Coumailleau P,Whitelaw ML,Gustafsson JA,Poellinger L||The Journal of biological chemistry (270:31353)||1995|
A tyrosine kinase-dependent pathway regulates ligand-dependent activation of the dioxin receptor in human keratinocytes.
MA3-011 was used in immunoprecipitation to investigate the role of a tyrosine kinase-dependent pathway during ligand-dependent activation of the dioxin receptor in human keratinocytes
|Gradin K,Whitelaw ML,Toftgård R,Poellinger L,Berghard A||The Journal of biological chemistry (269:23800)||1994|
Retrograde transport of the glucocorticoid receptor in neurites requires dynamic assembly of complexes with the protein chaperone hsp90 and is linked to the CHIP component of the machinery for proteasomal degradation.
MA3-011 was used in immunocytochemistry to study the role of Hsp90 and CHIP in the retrograde transport of the glucocorticoid receptor in neurites
|Galigniana MD,Harrell JM,Housley PR,Patterson C,Fisher SK,Pratt WB||Brain research. Molecular brain research (123:27)||2004|
KF25706, a novel oxime derivative of radicicol, exhibits in vivo antitumor activity via selective depletion of Hsp90 binding signaling molecules.
MA3-011 was used in western blot to investigate the mechanism of KF25706 for its in vivo antitumor activity
|Soga S,Neckers LM,Schulte TW,Shiotsu Y,Akasaka K,Narumi H,Agatsuma T,Ikuina Y,Murakata C,Tamaoki T,Akinaga S||Cancer research (59:2931)||1999|
The benzoquinone ansamycin 17-allylamino-17-demethoxygeldanamycin binds to HSP90 and shares important biologic activities with geldanamycin.
MA3-011 was used in western blot to investigate the effect of 17-allylamino-17-demethoxygeldanamycin on heat shock protein 90 chaperone function
|Schulte TW,Neckers LM||Cancer chemotherapy and pharmacology (42:273)||1998|
Hsp90, hsp4, HSP89A, AL024147, RCJMB04_28d6, HSPCA, HSPN, AL024080, HSP90A, HSPCAL4, EL52, HSP86, HSPCAL1, Hsp86-1, 86kDa, HSP90N, Hsp89, HSPC1, 89kDa, Hspca, Hsp86, LAP2
heat shock protein HSP 90-alpha, heat shock protein HSP90 alpha, hypothetical protein, HSP 86, epididymis luminal secretory protein 52, heat shock 86 kDa, heat shock 90kD protein 1, alpha, heat shock 90kD protein 1, alpha-like 4, heat shock 90kD protein, alpha-like 4, heat shock 90kDa protein 1, alpha, renal carcinoma antigen NY-REN-38, heat shock protein 1, alpha, heat shock protein 86, HSP86, TSTA, heat shock protein 90kDa alpha (cytosolic), class A member 1, heat shock protein, 1, heat shock protein, 86 kDa 1, heat shock protein, 89 kDa, tumor-specific transplantation 86 kDa antigen