|Tested species reactivity||Bovine, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Peptide corresponding to the EEVHHGEEEVEC sequence within N-terminal part of human Hsp90.|
|Storage buffer||PBS with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1 ug/ml|
|Immunoprecipitation (IP)||1 ug/ml|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes the EEVHHG epitope within the N-terminal part of Hsp90 beta, a ubiquitously expressed protein with calculated MW of 83.3 kDa which migrates, however, as a 90 kDa band under reducing SDS-PAGE conditions.
Hsp90 beta (heat shock protein 90 beta) is a constitutively expressed isoform of Hsp90, one of the most abundant chaperones in the cytosol of eukaryotic cells. Hsp90 interacts with various proteins, including protein kinases and transcription factors, and either facilitates their stabilization and activation or directs them for proteasomal degradation. Hsp90 thus affects multiple signaling pathways and biological processes and modulation of this single target offers the prospect of simultaneous intervence to various key points of oncogenic transformation. Hsp90 operates as a dimer in a conformational cycle driven by ATP binding and hydrolysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.