|Immunofluorescence (IF)||1:50 - 1:200|
|Immunoprecipitation (IP)||1:500 - 1:1000|
|Western Blot (WB)||1:200 - 1:1000|
|Immunoprecipitation (IP)||See 25 publications below|
|Western Blot (WB)||See 10 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|ELISA (ELISA)||See 2 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Tested Species reactivity||Chicken, Fish, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Rabbit , Rat , Non-human primate , Virus , Fruit fly , Human Mouse|
|Host / Isotype||Mouse / IgM|
|Immunogen||Purified mouse HSP90.|
|Storage buffer||ascites with Ammonium sulfate|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-011 detects heat shock protein 90 kDa (HSP90) from human, mouse, rat, rainbow trout, and chicken tissues.
MA3-011 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 90 kDa protein representing HSP90 from Hepa 1 cell extract. It has been reported that up to 90% of cytosolic HSP90 can be immunoprecipitated with this product under low ionic strength conditions.
For Western blots using MA3-011, nitrocellulose is recommended. PVDF membranes typically do not yield acceptable results with this antibody.
Before use, centrifuge suspension (4000 g for 15 minutes) and discard supernatant. Resuspend pellet in 200µl of PBS, pH 7.2. If desired, 0.05% sodium azide may be added to resuspended solution. Removal of residual ammonium sulfate is not usually necessary for immunoprecipitation and Western blot procedures, however, any other uses may require its removal by dialysis or equivalent method. Stable for 6 months after PBS resuspension.
The MA3-011 antigen is purified HSP90 from Hepa 1 cytosol.
Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: alpha; alpha-like 4; epididymis luminal secretory protein 52; epididymis secretory sperm binding protein Li 65p; Heat shock 86 kDa; heat shock 90kD protein; heat shock 90kD protein 1; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1; heat shock 90kDa protein 1, alpha; heat shock protein 1, alpha; heat shock protein 86; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha family class A member 1; Heat shock protein HSP 90-alpha; heat shock protein HSP90 alpha; heat shock protein, 1; heat shock protein, 86 kDa 1; heat shock protein, 89 kDa; HSP 86; HSP86; Hsp89; HSP89A; Hsp90; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; hypothetical protein; LAP-2; LAP2; Lipopolysaccharide-associated protein 2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38; TSTA; Tumor-specific transplantation 86 kDa antigen
Gene Aliases: 86kDa; 89kDa; AL024080; AL024147; EL52; HEL-S-65p; hsp4; HSP86; Hsp86-1; Hsp89; HSP89A; Hsp90; HSP90A; HSP90AA1; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP-2; LAP2; RCJMB04_28d6
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