Western blot analysis of HSPA2/Heat Shock 70 Protein 2 using 30µg of A) 293T (B) A431 (C) H1299 (D) HeLa (E) HepG2 and F) MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a HSPA2/Heat Shock 70 Protein 2 polyclonal antibody (Product # PA5-29038) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 365 and 639 of Human HSPA2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29038 targets HSPA2 in WB applications and shows reactivity with Human samples.
The PA5-29038 immunogen is recombinant fragment corresponding to a region within amino acids 365 and 639 of Human HSPA2.
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
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