Immunofluorescent analysis of HSPA6/Heat Shock 70 Protein 6 in methanol-fixed A431 cells using a HSPA6/Heat Shock 70 Protein 6 polyclonal antibody (Product # PA5-31480) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:500.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 233 and 536 of Human HSPA6|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31480 targets HSPA6 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-31480 immunogen is recombinant fragment corresponding to a region within amino acids 233 and 536 of Human HSPA6.
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.