Immunofluorescent analysis of A2058 cells using a HSPA8/Heat Shock 70 Protein 8 polyclonal antibody (Product # PA5-24624) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Actin filaments were stained with dye-conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
|Tested species reactivity||Human|
|Published species reactivity||Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 82-110 amino acids from the N-terminal region of human HSPA8|
|Purification||Ammonium sulfate precipitation, Size-exclusion - Dialysis|
|Contains||0.09% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with bovine, chicken, hamster, mouse and rat based on sequence homology.
HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. The protein also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Non-human primate||Not Cited||
Multi-faceted proteomic characterization of host protein complement of Rift Valley fever virus virions and identification of specific heat shock proteins, including HSP90, as important viral host factors.
PA5-24624 was used in western blot to perform a proteomic analysis of host proteins in Rift Valley fever virus virions and to study the particular significance of Hsp90
|Nuss JE,Kehn-Hall K,Benedict A,Costantino J,Ward M,Peyser BD,Retterer CJ,Tressler LE,Wanner LM,McGovern HF,Zaidi A,Anthony SM,Kota KP,Bavari S,Hakami RM||PloS one (9:null)||2014|