Western blot analysis of HSPB8/Heat Shock Protein beta 8 in human Muscle lysate (RIPA buffer, 30g total protein per lane) using a HSPB8/Heat Shock Protein beta 8 polyclonal antibody (Product # PA1-31842) at a dilution of 1g/ml following incubation for 1 hour and detected using chemiluminescence.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence NELPQDSQEVTCT, from C Terminus of the protein sequence according to NP_055180|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31842 detects HSPB8 from human samples.
PA1-31842 has been successfully used in Western blot applications.
The PA1-31842 immunogen is: Peptide with sequence NELPQDSQEVTCT, from C Terminus of the protein sequence according to NP_055180
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.