Immunofluorescent analysis of HSPE1/Cpn10 showing staining in the mitochondria of SKNSH cells. SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 15 min and stained using a HSPE1/Cpn10 polyclonal antibody (Product # PA5-30428) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 1 and 52 of Human Cpn10|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30428 targets Cpn10 in WB applications and shows reactivity with mouse samples.
The PA5-30428 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 52 of Human Cpn10.
This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2.
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