Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 8
HSPH1 Antibody (MA5-36231) in ICC/IF
Immunocytochemistry analysis of HSPH1 in MCF7 cells. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in HSPH1 monoclonal antibody (Product # MA5-36231) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.
Product Details
MA5-36231
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3D10
Immunogen
E. coli-derived human Hsp105 recombinant protein (Position: Y653-D858).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2884065
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
HSPH1 prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. This protein inhibits HSPA8/HSC70 ATPase and chaperone activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Antigen NY-CO-25; heat shock 105kD alpha; heat shock 105kD beta; heat shock 105kDa/110kDa protein 1; Heat shock 110 kDa protein; Heat shock protein; Heat shock protein 105 kDa; Heat shock protein family H member 1; HSP; NY CO 25
Gene Aliases: HSP105; HSP105A; HSP105B; HSP110; HSPH1; KIAA0201; NY-CO-25
UniProt ID: (Human) Q92598
Entrez Gene ID: (Human) 10808
receptor-mediated endocytosis
response to unfolded protein
positive regulation of MHC class I biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
chaperone mediated protein folding requiring cofactor
positive regulation of NK T cell activation
positive regulation of protein tyrosine kinase activity
regulation of cellular response to heat
negative regulation of establishment of protein localization to mitochondrion
negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide
negative regulation of p38MAPK cascade
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support