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HYB 133-09-02 has specificity for native human factor IX.
HYB 133-09-02 has been successfully used in ELISA and Western blot procedures. HYB 133-09-02 reacts strongly with native factor IX. Strong reaction is seen in ELISA with native factor IX directly coated onto the microtiter plate. In sandwich ELISA it can be used in combination with a polyclonal antibody against factor IX. In Western blotting after SDS-PAGE, HYB 133-09-02 reacts with factor IX in reduced as well as non-reduced form.
The HYB 133-09-02 immunogen is Factor IX isolated from human plasma.
Epitope specificity of HYB 133-09-02 differs from that of HYB 133-01 as determined by inhibition ELISA.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Coagulation factor IX is a single chain polypeptide with a molecular mass of 57 kDa. Normal concentrations of factor IX in human plasma is approximately 3 µg/mL. Factor IX deficiency and lack of coagulation activity is seen in patients suffering from the innate X-chromosome linked disorder, haemophilia B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Christmas factor; Coagulation factor IX; Coagulation factor IXa heavy chain; Coagulation factor IXa light chain; EC 3.4.21.22; factor 9; factor IX F9; Factor9; FIX F9; plasma thromboplastic component; Plasma thromboplastin component; PTC; RP6-88D7.1; serine protease
Gene Aliases: F9
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