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Invitrogen
HYB 190-01-02 is specific for brain AChE and does not recognize AChE from erythrocytes. The antibody can thus distinguish between mammalian brain AChE and eruthrocyte AChE. Weak cross-reactivity with Torpedo marmorata AChE but none with AChE from electric eel or human BtChE.
HYB 190-01-02 has been successfully used in ELISA and Western blotting techniques. Can be used in ELISA on amniotic fluid for the diagnosis of neural tube defects. HYB 190-01-02 is well suited as catching antibody (on an anti-mouse IgG coat) in enzyme antigen immunoassay (EAIA), where the antigen (AChE) is captured and used directly as substrate for acetylthiocholiniodide (Ellmann's reaction). In Western blotting and dot blotting HYB 190-01-02 reacts with native and denatured human and bovine, detergent soluble and salt-soluble AChE. No cross-reactivity is seen with erythrocyte AChE.
The HYB 190-01-02 immunogen is the C-terminal 10 residues of brain acetylcholinesterase (human and bovine), absent from the eruthrocyte enzyme. Coupled to PPD with glutaraldehyde.
The epitope of HYB 190-01-02 is C-terminal 10 residues (aa 574-583) of brain acetylcholinesterase (DS-AChE and SS-AChE).
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. The Protein is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Acetylcholinesterase; acetylcholinesterase (Yt blood group); acetylcholinesterase T-subunit; AChE; apoptosis-related acetylcholinesterase; EC 3.1.1.7; OTTHUMP00000211349; OTTHUMP00000211356; Yt blood group
Gene Aliases: ACHE
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