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Hakai Antibody (A302-969A) in WB
Detection of human and mouse Hakai by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Hakai antibody (Product # A302-969A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. Hakai was also immunoprecipitated by rabbit anti-Hakai antibody A302-968A, which recognizes an upstream epitope. Detection: Chemiluminescence with expo... View More
Product Details
A302-969A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 441 and 491 of human E3 ubiquitin-protein ligase Hakai.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
This gene encodes an E3 ubiquitin-ligase for the E-cadherin complex and mediates its ubiquitination, endocytosis, and degradation in the lysosomes. The encoded protein contains a RING-finger domain and is also thought to have a role in control of cell proliferation. A related pseudogene has been identified on chromosome X. Alternative splicing results in a non-coding transcript variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-Cbl-like protein 1; Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1; Casitas B-lineage lymphoma-transforming sequence-like protein 1; Cbl proto-oncogene like 1, E3 ubiquitin protein ligase; Cbl proto-oncogene, E3 ubiquitin protein ligase-like 1; Cbl proto-oncogene-like 1, E3 ubiquitin protein ligase; E-cadherin binding protein E7; E3 ubiquitin-protein ligase Hakai; RING finger protein 188; RING-type E3 ubiquitin transferase Hakai
Gene Aliases: AI467391; c-Cbl-like; CBLL1; HAKAI; RNF188
UniProt ID: (Human) Q75N03, (Mouse) Q9JIY2
Entrez Gene ID: (Human) 79872, (Mouse) 104836
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
identical protein binding
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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