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Chromotek
Halo VHH Recombinant Alpaca Monoclonal Antibody (CTK0213)
Product Details
OT-250
Product Specifications
Species Reactivity
Tag
Host/Isotype
Alpaca
Expression System
E. coli
Class
Recombinant Monoclonal
Type
Antibody
Clone
CTK0213
Immunogen
Halo protein from Branchiostoma lanceolatum
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
25mM TAPS, pH 8.5, with 5mM EDTA, 500mM NaCl
Contains
0.09% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Wet ice
Product Specific Information
Anti-Halo Nanobody for conjugation to beads, plates and surfaces via NHS-chemistry.
Affinity: Dissociation constant KD of 2 nM. Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies).
Target Information
The HaloTag is a self-labeling protein derived from the haloalkane dehalogenase enzyme DhaA from Rhodococcus rhodochrous. It can be used as protein tag for tagging your protein of interest (POI). After transfection of your cell line/organism with a plasmid encoding for your Halo-tagged protein, the Halo-tagged protein is expressed. Through incubation of your cell line/organism with specific ligands (see below point 6), your Halo fusion protein turns into a fluorescent protein by binding a fluorescent dye or acquires biotin for immobilization or other streptavidin modifications. Once the HaloTag has formed a complex with a ligand, the catalytic center is not accessible for additional ligands. Alternatively, the Halo fusion protein can be detected or immunoprecipitated by antibodies or Nanobodies binding outside of its catalytic center. The HaloTag consists of 297 amino acids and has a molecular weight of 33 kDa. The HaloTag is derived from haloalkane dehalogenase enzyme DhaA. Its active site has been genetically modified. Chloroalkane linker substrates are irreversibly bound to the active site.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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