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Western blot analysis of mammalian ATG9 (mATG9) was performed by loading the indicated amounts of control or human ATG9 overexpression lysates onto a 4-12% Bis-Tris polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA in TBST for at least 1 hour. Membranes were probed with an ATG9 monoclonal antibody (Product # MA1-149) at a dilution of 1:500 overnight at 4°C on a rocking platform, washed in TBST, and probed with an HRP-conjugated goat anti-hamster IgG secondary antibody (Product # PA1-29626) at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
|Tested species reactivity||Hamster|
|Host / Isotype||Goat / IgG|
|Immunogen||Full length native Syrian hamster IgG (purified).|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/ml BSA|
|Contains||0.01% gentamicin sulfate|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:15,000 - 1:65,000|
|Immunohistochemistry (Frozen) (IHC (F))||1:2500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2500|
|Western Blot (WB)||1:15,000 - 1:65,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29626 detects Hamster IgG from Syrian hamster samples.
PA1-29626 has been successfully used in ELISA, immunohistochemistry (frozen tissue), Western blot, and immunohistochemistry (paraffin tissue) applications.
The PA1-29626 immunogen is full length native Syrian hamster IgG (purified).
Thermo Scientific Anti-Hamster secondary antibodies are affinity-purified antibodies with well-characterized specificity for hamster immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.