|Tested species reactivity||Bacteria|
|Published species reactivity||Bacteria|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified Helicobacter pylori.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is Helicobacter pylori infected tissue.
Helicobacter pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcimona and low grade lymphoma of mucosa associated lymphoid tissue in the stomach.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mechanism of H. pylori intracellular entry: an in vitro study.
PA5-32442 was used in immunohistochemistry to study intracellular entry of Helicobacter pylori and the role played by the nudA invasin
|Liu H,Semino-Mora C,Dubois A||Frontiers in cellular and infection microbiology (2:null)||2012|