|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Rat heme oxygenase 1.|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Inhibition Assays (IA)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||5-10 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
|Western Blot (WB)||5-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA1-25475 detects Heme Oxygenase 1 from human, mouse, and rat samples. MA1-25475 will not react with rabbit.
MA1-25475 has been successfully used in ELISA, inhibition assay, immunohistochemistry, and Western blot procedures.
The MA1-25475 immunogen is rat heme oxygenase 1.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Adaphostin toxicity in a sensitive non-small cell lung cancer model is mediated through Nrf2 signaling and heme oxygenase 1.
MA1-25475 was used in western blot to investigate the molecular mechanisms of adaphostin toxicity.
|Fer ND,Shoemaker RH,Monks A||Journal of experimental and clinical cancer research : CR (29:null)||2010|