Immunofluorescent analysis of Heme Oxygenase-1 (HO-1, green) in HeLa cells either left untreated (left panel) or treated with 0.5mM copper sulfate (right panel) for 24 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an HO-1 monoclonal antibody (Product # MA1-112) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Bovine, Dog, Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to the sequence near the N-terminus (amino acids 1-30) of human Heme Oxygenase 1|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.09% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||10 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
This antibody detects an ~32 kDa protein corresponding to the apparent molecular mass of HO-1 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine and dog origins. This antibody does not cross-react with HO-2.
This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Anti-fatigue effect of Myelophil in a chronic forced exercise mouse model.
MA1-112 was used in western blot to assess the anti-fatigue effects of Myelophil.
|Lee JS,Kim HG,Han JM,Kim YA,Son CG||European journal of pharmacology (764:100)||2015|
The traditional drug Gongjin-Dan ameliorates chronic fatigue in a forced-stress mouse exercise model.
MA1-112 was used in western blot to evaluate the anti-fatigue effects of Gongjin-Dan in a chronic forced exercise mouse model
|Hong SS,Lee JY,Lee JS,Lee HW,Kim HG,Lee SK,Park BK,Son CG||Journal of ethnopharmacology (168:268)||2015|