Immunofluorescent analysis of Heme Oxygenase 1 showing staining in the nucleus and cytoplasm of A375 cells. A375 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Heme Oxygenase 1 polyclonal antibody (Product # PA5-27338) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 278 of Human Heme Oxygenase 1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% Proclin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA5-27338 targets Heme Oxygenase 1 in IHC (P) and WB applications and shows reactivity with Human and mouse samples.
The PA5-27338 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 278 of Human Heme Oxygenase 1.
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hemophagocytosis-mediated keratinization in oral carcinoma in situ and squamous cell carcinoma: a possible histopathogenesis of keratin pearls.
PA5-27338 was used in immunohistochemistry to study oral carcinoma in situ and squamous cell carcinoma keratinization and the role of erythro-hemophagocytosis in the formation of keratin pearls
|Al-Eryani K,Cheng J,Abé T,Yamazaki M,Maruyama S,Tsuneki M,Essa A,Babkair H,Saku T||Journal of cellular physiology (228:1977)||2013|